Journal ArticleDOI
Transient expression of marker genes in immature zygotic embryos of spring wheat (Triticum aestivum) through microprojectile bombardment
Reads0
Chats0
TLDR
Transient expression of marker genes (cat and uidA) delivered by the Biolistics microprojectile bombardment technique has been detected in immature zygotic embryos of wheat.Abstract:
Transient expression of marker genes (cat and uidA) delivered by the Biolistics microprojectile bombardment technique has been detected in immature zygotic embryos of wheat (Triticum aestivum L.). The DNA expression vectors that gave maximal expression of both cat (pCaMVI1CN) and uidA (pCaMVI1GusN) genes had an alcohol dehydrogenase (Adh1) intron 1 cloned in between the cauliflower mosaic virus (CaMV35S) promoter and the coding region of the gene. Detection of chloramphenicol acetyltransferase (CAT) activity in response to cat gene was complicated by the presence of an inhibitor of CAT activity as well as an endogenous CAT-Iike activity. The results of enzymatic assays were confirmed by an ELISA technique using CAT-specific antibodies, whereas the β-glucuronidase (GUS) activity following the introduction of the uidA gene was confirmed by both histochemical and fluorometric techniques.Key words: cereal transformation, gene expression, ELISA, wheat.read more
Citations
More filters
Journal ArticleDOI
Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus
TL;DR: Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus as mentioned in this paper, which can be used to produce wheat.
Patent
Methods for stable transformation of wheat
TL;DR: In this paper, a method for the production of fertile transformed wheat plants was described, which involved subjecting wheat tissues to high velocity microprojectile bombardment, selecting for transformed cells, and regenerating stably transformed fertile plants from the transformed cells.
Journal ArticleDOI
Recent advances in the malting and brewing industry
TL;DR: In this paper, the authors present a system for continuous main fermentation with immobilized yeast, where the residence time in the bioreactor is only 2 h, whereas several weeks are needed for traditional lagering.
Journal ArticleDOI
Wheat biotechnology: A minireview
Debasis Patnaik,Paramjit Khurana +1 more
TL;DR: Use of marker assisted selection and genomics approaches will increase the effectiveness and efficiency of wheat breeding programs, and will also provide insights into genetic control of key traits to be used for genetic manipulation.
Journal ArticleDOI
Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.)
TL;DR: A dramatic dependence on the presence of an intron for chimeric gene expression in both maize and bluegrass is confirmed and the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots.
Related Papers (5)
Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more