Validasi Metode Penetapan Kadar Dehidrolovastatin Dalam Plasma In Vitro Dengan KCKT
Gogok Hariyanto,L. Broto Sugeng Kardono,Umar Mansyur +2 more
- Vol. 5, Iss: 2, pp 160926
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TLDR
The aim of this study was to validate method forin vitro analysis of dehidrolovastatin in plasm, which included studies of calibration curve and linearity, LLOQ and selectivity, accuracy, precision, recovery, and stability.Abstract:
Statins are antihyperlipidemic drugs for lowering LDL-cholesterol level in human blood. They were designed to inhibit HMG CoA reductase in the liver so that the enzyme will not catalyze the transformation of HMG CoA into early precursor of LDL-cholesterol. Dehydrolovastatin is a kind of statins whose structure is analogous to lovastatin (its starting material). The aim of this study was to validate method forin vitro analysis of dehidrolovastatin in plasm. The validation included studies of calibration curve and linearity, LLOQ and selectivity, accuracy, precision, recovery,and stability. Dehidrolovastatin was deteminated by Knauer r HPLC using UV 2500 detector, Kromasil r 100-5, C18, 250 mm, 4.6 mm i.d., column. Reversed phase was applied with the optimal condition such as mobile phase acetonitrile and phosphoricacid 0.1 % (75:25), the flow rate of 1.2 mL. minutesn -1, simvastatin as internal standard and wavelength 238 nm. Concentrations of sample ranged from 0.013 to0.200 ppm with correlation coefficient of the calibration curves 0,998 and lower limit of quantitation was 0.013 ppm. The results of validation studies fulfilled standard criteria.read more
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The Analysis of Drugs in Biological Fluids
TL;DR: This work analyzes drugs in Biological Fluids using Spectroscopy, Fluorimetry, Gas Chromatography, and Other Ligand Assays for the determination ofaturation levels in response to various Structures of Drugs.
Journal ArticleDOI
Fermentation optimization for the production of lovastatin by Aspergillus terreus: use of response surface methodology
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,F.G. Acién Fernández,E. Molina Grima,Yusuf Chisti +5 more
TL;DR: A Box-Behnken experimental design was used to investigate the effects of five factors (oxygen content in the gas phase, concentrations of C, N and P, and fermentation time) on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus as discussed by the authors.
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Small Intestinal Metabolism of the 3-Hydroxy-3-methylglutaryl-Coenzyme A Reductase Inhibitor Lovastatin and Comparison with Pravastatin
Wolfgang Jacobsen,Gabriele I. Kirchner,Katrin Hallensleben,Laviero Mancinelli,M. Deters,Ingelore Hackbarth,Karen Baner,Leslie Z. Benet,Karl-Friedrich Sewing,Uwe Christians +9 more
TL;DR: It was concluded that lovastatin is metabolized by cytochrome P-450 3A enzymes in the small intestine and cannot be expected to significantly affect its oral bioavailability or to be a significant site of drug interactions.
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Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere
TL;DR: In this article, a simple capillary electrophoresis (CE) method for Lovastatin determination was reported, which is selective with respect to its degradation products and useful for routine analyses.
Journal ArticleDOI
A comparison of the effects of lovastatin and pravastatin on ubiquinone tissue levels in rats
TL;DR: Findings may indicate that the use of some HMG-CoA reductase inhibitors may not be appropriate in patients with preexisting pathologic conditions associated with decreased levels of ubiquinones, both as a liposoluble antioxidant and in mitochondrial bioenergetics.