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Can Nofap increase sperm count?

Male infertility

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Journal Article•DOI A novel microfluidic device for selecting human sperm to increase the proportion of morphologically normal, motile sperm with uncompromised DNA integrity Bin Zhang, Tailang Yin, Jun Yang - Show less +2 more 09 Jul 2015-Analytical Methods 17 Citations	This method can not only minimize damage to the sperm structure, but also increase the effectiveness of selection.
Open access•Journal Article•DOI Conservative Nonhormonal Options for the Treatment of Male Infertility: Antibiotics, Anti-Inflammatory Drugs, and Antioxidants. Aldo E. Calogero, Rosita A. Condorelli, Giorgio Ivan Russo, Sandro La Vignera - Show less +3 more 09 Jan 2017-BioMed Research International 52 Citations	Combined therapies can contribute to improve sperm quality.
Open access•Journal Article•DOI Evaluation of human sperm function after repeated freezing and thawing. Enoka Bandularatne, Ariff Bongso - Show less +1 more 04 Mar 2002-Journal of Andrology 23 Citations	This approach has tremendous benefits for men who have difficulty producing sperm and for those with low and declining sperm counts.
Journal Article•DOI Propecia-induced spermatogenic failure: a report of two cases. Kimberly E. Liu, Saleh Binsaleh, Kirk C. Lo, Keith Jarvi - Show less +3 more 01 Sep 2008-Fertility and Sterility 47 Citations	(S) Improvement in sperm concentration.
Open access•Journal Article•DOI Demonstration of the effect of epidermal growth factor on ram sperm parameters using two fluorescent assays. A. V. Makarevich, E. Kubovičová, A. V. Sirotkin, J. Pivko - Show less +3 more 11 Feb 2018-Veterinarni Medicina 12 Citations	These findings suggest that the technique chosen for analysis of sperm viability can influence the conclusion concerning the effects of the treatment on sperm function.

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Issues with ctab lysis buffer

The use of CTAB lysis buffer in DNA extraction processes can present various challenges. While CTAB is commonly employed for efficient DNA extraction from plants, issues arise when considering its impact on DNA purity and quality. Factors such as incubation temperature and lysis duration can influence DNA yield and fragment lengths, with purity being significantly affected. Additionally, the presence of 2-mercaptoethanol (2-ME) in lysis buffer can interfere with DNA extraction from certain microorganisms, leading to poor DNA integrity. To ensure high-quality DNA for molecular analyses, alternative purification approaches like gradient or cell sorting-based methods may be more effective in eliminating non-sperm cell contaminants in sperm samples, as traditional methods like somatic cell lysis buffer treatment may result in substantial cell loss without enhanced purification.Why is parameter of Linearity sperm in spermogram importment?

The parameter of linearity in spermograms is crucial as it correlates with sperm mobility and fertility potential. Studies show that specific sperm velocity parameters like straight-line velocity (VSL), linearity (LIN), and beat-cross frequency (BCF) are significantly associated with sperm mobility in turkeys. Additionally, research on mouse sperm motility distinguishes between linear and nonlinear tracks, highlighting the importance of linear movement in sperm function. Furthermore, the low variation in sperm motion parameters, including linearity, suggests that computerized motion analysis of sperm can detect subtle effects of environmental toxins on reproductive functions in men, emphasizing the significance of assessing linearity in sperm health evaluations. Therefore, evaluating linearity in spermograms provides valuable insights into sperm function and fertility potential across different species.Why therapy has a really low percent of effectiveness on men?

Therapy's low effectiveness on men can be attributed to various factors. Cultural, socialization, and gender-related issues contribute to African American males' low participation in therapy, highlighting the impact of societal norms on help-seeking behaviors. Additionally, traditional therapy models may inadvertently reinforce gender-role stereotypes, hindering effective treatment for men. Young adult men, particularly in their late teens and early twenties, face significant challenges with emotional management and interpersonal relationships, making therapy complex and challenging despite its potential effectiveness when tailored appropriately. Moreover, pessimism surrounding therapy for men stems from historical limitations in treating subfertile males, leading to missed opportunities for meaningful interventions in this group. These combined factors underscore the need for tailored approaches that address men's unique psychological and social needs to enhance therapy effectiveness.Has ATR inhibition ever been tested in glioblastoma cells and inflammation ?

ATR inhibition has been investigated in glioblastoma cells, showing promising results. Studies have demonstrated that ATR inhibition reduces invasion of GBM cells by impacting cytoskeletal networks and integrin trafficking, suggesting a potential therapeutic target for GBM invasion. Additionally, ATR inhibition in glioma cells resulted in reduced viability and clonogenic survival, indicating anti-tumor effects. Furthermore, combining ATR inhibition with radiation therapy in HPV-driven malignancies led to significant radiosensitization and increased immune cell infiltration, particularly myeloid cells, triggering an IFN response and enhancing antigen presentation within the tumor microenvironment. These findings highlight the potential of ATR inhibition in glioblastoma treatment and its impact on inflammation within the tumor microenvironment.What is the current understanding of the role of the microbiome in fertility in humans?

The microbiome plays a crucial role in human fertility by influencing various aspects of the reproductive system. In males, changes in the gut microbiome can impact systemic sex hormones and spermatogenesis, potentially affecting male fertility. In females, the presence of different microorganisms in the reproductive tract can regulate fertility, with a Lactobacillus-dominated uterine environment associated with better pregnancy outcomes. Dysbiosis in the female reproductive microbial communities can lead to fertility issues and impact assisted reproductive procedures like in vitro fertilization. Similarly, in cattle, the reproductive tract microbiome interacts with the uterine environment to maintain health and fertility, with potential for manipulation through diet changes and probiotics to improve reproductive outcomes. Probiotics, particularly Lactobacillus, show promise in mitigating the negative impact of pathogenic bacteria on male fertility parameters.How does the composition of the semen microbiome vary between fertile and infertile bulls?

The composition of the semen microbiome differs between fertile and infertile bulls. Research using 16S sequencing on healthy bulls identified 107 bacterial genera in semen, with variations noted between individual bulls. Negative correlations were observed between certain bacterial genera and bull fertility, such as Curvibacter, Rikenellaceae RC9-gut-group, and Dyella spp. Additionally, bacteria like Cutibacterium, Ruminococcaceae UCG-005, and Staphylococcus showed negative correlations with fertility. Enrichment of W5053 and Lawsonella genera was found in bulls with low fertility, indicating a potential association between specific bacterial profiles and bull fertility status. Furthermore, in infertile men with non-obstructive azoospermia, differences in the semen microbiota were observed compared to fertile controls, with underrepresentation of Proteobacteria and Firmicutes and overrepresentation of Actinobacteriota at the phylum level.Why cancer genes are important for spermatogenesis?

Cancer genes are crucial for spermatogenesis due to their shared expression patterns and functional similarities between the two processes. The identification of cancer/testis (CT) genes, which are normally restricted to the testis, has provided valuable insights into both tumor growth and spermatogenesis. These genes, expressed during spermatogenesis, play essential roles in cell division, migration, and immortality, processes vital for both cancer development and sperm production. Furthermore, the discovery of germ cell-specific cancer-expressed genes has highlighted their potential as targets for cancer therapy, with the ability to inhibit tumor growth while potentially impacting fertility. Understanding the overlap between cancer and spermatogenesis at the genetic level opens up avenues for novel cancer treatments and sheds light on the intricate processes involved in both contexts.Does stress affect reproductive investment time in insects?

Stress can indeed impact reproductive investment time in insects. Research on butterflies like Pieris napi and Speyeria mormonia suggests that stressful developmental conditions can lead to reduced adult body size, altered reproductive output, and changes in metabolic rates. In Anopheles coluzzii mosquitoes, hydric stress influences male reproductive effort by increasing sperm transfer to females, while females maintain sperm quality despite stress, optimizing their reproductive value. Hormones like dopamine, adipokinetic hormone, juvenile hormone, and ecdysteroids play crucial roles in coordinating growth and fecundity responses to stress in insects. Therefore, stress can significantly impact the timing and allocation of reproductive resources in insect populations, highlighting the intricate interplay between environmental stressors and reproductive strategies.What are the potential problems associated with mutations in the HE strand?

Mutations in the HE strand, specifically in the HESX1 gene, can lead to growth hormone deficiency (GHD) and a range of associated anomalies. Additionally, mutations in genes like MYH can cause polyposis syndrome, characterized by colorectal adenomas and cancer. In the context of male infertility, chromosomal aberrations and mutations in genes responsible for male germ cell development can contribute to reproductive defects. These mutations can result in complex medical problems, with male infertility being a significant concern due to its frequency and heterogeneous causes. Therefore, understanding and identifying mutations in genes like HESX1, MYH, and others are crucial for diagnosing and managing associated disorders and conditions.Why Circular tracks sperm is important in spermogram?

Circular tracks sperm movement is crucial in spermograms due to its correlation with fertilization success. Circular movement patterns in sperm, especially in broadcast spawning species like Mytilus, enhance fertilization by increasing gamete interaction rates and potentially aiding in avoiding swimming away from eggs prematurely. Additionally, circular RNA circ_MGLL has been linked to sperm retrieval outcomes in patients with idiopathic non-obstructive azoospermia (iNOA) undergoing microdissection testicular sperm extraction (micro-TESE). Circular trace reconstruction studies have shown that circular strings can be reconstructed efficiently, which could have implications in understanding genetic material related to sperm characteristics. Therefore, understanding circular tracks in sperm movement and genetic circular structures can provide valuable insights into fertility and reproductive outcomes.What factors influence the reproductive success and survival of bird whelps in the wild?

Factors influencing the reproductive success and survival of bird whelps in the wild include social familiarity, immune gene variation, sperm traits, and fitness proxies. Social familiarity with neighbors and breeding partners positively impacts reproductive success. Immune gene variation affects adult longevity, with high MHC allele diversity decreasing longevity. Sperm quantity, rather than morphology, influences male reproductive success, especially within-pair paternity success. Short-term fitness proxies, such as annual fitness at one year old, can predict long-term fitness when measured at the recruitment stage. These findings highlight the complex interplay of social interactions, genetic factors, and fitness metrics in shaping the reproductive success and survival of bird whelps in the wild.