Can the identification and characterization of cell wall degrading enzymes produced by Botrytis?
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Yes, the identification and characterization of cell wall degrading enzymes produced by Botrytis cinerea, a necrotrophic fungal pathogen, have been extensively studied through various proteomic and genomic approaches. Botrytis cinerea is known for its ability to infect a wide range of host plants, primarily through the secretion of enzymes that degrade plant cell walls, facilitating host penetration and colonization. Proteomic analysis has revealed a significant number of proteins secreted by B. cinerea when grown on different carbon sources, including those involved in the degradation of pectin, a major component of the plant cell wall. Specifically, the genome of B. cinerea harbors genes encoding for a variety of Carbohydrate-Active enZymes (CAZymes), which are crucial for the disassembly of cell wall polysaccharides. Among these, pectin-degrading enzymes, such as endopolygalacturonases and pectin methylesterases, play a pivotal role in the infection process by facilitating the penetration of the plant surface and growth into the middle lamella. Further studies have identified and characterized specific CAZymes, including those involved in the degradation of cellulose and hemicellulose, highlighting the diversity of the Botrytis CAZymes and suggesting a selective attack on host wall polysaccharide substrates depending on the host tissue. Additionally, the functional analysis of mutants lacking certain cell wall-degrading enzyme genes has provided insights into the roles of these enzymes in virulence and pathogenicity. Moreover, the identification of proteins with SignalP motifs indicates the secretion of these enzymes into the extracellular space, where they can interact with plant cell walls. The expression of these enzymes during infection of various host plants has been confirmed through RNAseq, further establishing their importance in the pathogenicity of B. cinerea. In summary, the identification and characterization of cell wall-degrading enzymes produced by Botrytis cinerea have been achieved through comprehensive proteomic and genomic studies, elucidating their crucial roles in the infection process and offering potential targets for the development of new control strategies.
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| Papers (10) | Insight |
|---|---|
52 Citations | Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. |
16 Citations | Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. |
| Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. | |
| Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. | |
| Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. | |
75 Citations | Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. |
| Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. | |
| Yes, the paper discusses the molecular identification and functional characterization of endopolygalacturonases by Botrytis cinerea, crucial for pectin degradation and virulence mechanisms in plants. | |
| Not addressed in the paper. | |
30 Aug 2021 4 Citations | The research identifies signaling cascades and virulence factors of B. cinerea during tomato cell wall degradation, providing insights into potential cell wall degrading enzymes. |
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