Do N2A neuroblastoma cells have greater glutamate uptake than COS-7 cells?
Best insight from top research papers
N2A neuroblastoma cells have been shown to have a concentration-dependent response to L-glutamate, as well as other glutamate analogues, resulting in an inward current . These cells have also been used as a model for studying excitatory amino acid properties . However, there is no information available in the provided abstracts regarding the glutamate uptake in N2A neuroblastoma cells compared to COS-7 cells.
Answers from top 4 papers
More filters
Papers (4) | Insight |
---|---|
23 Citations | The provided paper does not mention N2A neuroblastoma cells or COS-7 cells. Therefore, there is no information in the paper to answer the question about the glutamate uptake of these specific cell types. The paper is about the presence of functional NMDA receptors in a human neuroblastoma cell line. |
5 Citations | The provided paper does not mention anything about the glutamate uptake of N2A neuroblastoma cells compared to COS-7 cells. |
The provided paper does not mention anything about the glutamate uptake of N2A neuroblastoma cells compared to COS-7 cells. The paper focuses on the evaluation of calcium oscillations and neural spikes in mouse neuroblastoma N2A cells in an epileptic condition. | |
124 Citations | The provided paper does not mention anything about the glutamate uptake of N2A neuroblastoma cells or COS-7 cells. |
Related Questions
What is the role of glutamate in neurons as an energetic substrate?5 answersGlutamate plays a crucial role in neuronal energy metabolism by serving as an energetic substrate. It can be utilized for ATP synthesis, especially during hypoxia/reoxygenation injury, where glutamate supplementation can improve cell survival and ATP levels. Glutamate influences neuronal energy metabolism by regulating the expression of key mitochondrial proteins like AQP9, MCT2, and LDHA. Additionally, the enzyme glutamate dehydrogenase (GDH) is essential for connecting amino acid, carbohydrate, neurotransmitter, and oxidative energy metabolism in neurons. Glutamate metabolism is intricately linked to the glutamate-glutamine cycle, highlighting its significance in sustaining glutamatergic activity and neurotransmission. Overall, glutamate serves as a vital energy substrate in neurons, contributing to their metabolic functions and survival under various conditions.
What is the relationship between GABA and Glutamate?5 answersThe relationship between GABA and Glutamate is crucial for maintaining physiological brain functions. Both neurotransmitters play significant roles in various processes, including the sleep-wake cycle. Glutamate is primarily involved in excitatory synaptic functions, plasticity, and excitotoxicity, while GABA underlies inhibition and can impact psychophysical functions. Studies have shown that disturbances in GABAergic and glutamatergic neurotransmission can lead to serious health issues, affecting neurodegeneration, affective disorders, and sleep problems like obstructive sleep apnea (OSA). In the context of glaucoma, a decline in GABA levels, rather than glutamate, has been linked to reduced neural specificity in the visual cortex, suggesting the importance of GABA in maintaining neural function. Additionally, changes in glutamate levels have been associated with motor learning, highlighting the intricate interplay between GABA and glutamate in cognitive processes.
Do extracellular glutamate levels depend on vesicular release of glutamate?5 answersExtracellular glutamate levels are influenced by vesicular release mechanisms. Studies suggest that glutamate release can occur through full fusion of vesicles with the membrane or via 'kiss-and-run' mechanisms, where glutamate is released through a fusion pore. During brain ischemia, the cystine/glutamate antiporter plays a crucial role in elevating extracellular glutamate levels, leading to neuronal damage through overactivation of extrasynaptic NMDA receptors. Research on cultured neurons indicates that glutamate release can deplete vesicular stores, with sustained release dependent on Ca2+ levels and vesicular mechanisms. Furthermore, vesicular filling of glutamate, removal of Synapsin I, and Ca2+-dependent exocytosis are key steps in the release process. Additionally, satellite glial cells have been shown to release glutamate in a calcium-dependent manner, with potential modulation by botulinum neurotoxin type A.
Does glutamate play a role in ALS?5 answersGlutamate plays a role in ALS. Increased extracellular glutamate in ALS and cerebral ischemia stimulates the utilization of glutamate as metabolic fuel, suggesting a beneficial role for glutamate in these conditions. Glutamatergic excitotoxicity or dysregulation of glutamatergic signaling is considered a mechanism underlying motor neuron death in ALS. Altered glutamate homeostasis is believed to be involved in ALS pathogenesis, and the approved treatment for ALS, riluzole, modulates glutamate. Glutamate metabolism disorders are connected to TDP-43 proteinopathy, a pathological mechanism in ALS. Glutamate-mediated corticomotor neuronal hyperexcitability is correlated with ALS, and disruption of the neuron-astrocyte glutamate-glutamine cycle may retard ALS progression.
Why when transfecting gene into Neuro-2a from the media not direct transfer still occurs?5 answersWhen transfecting genes into Neuro-2a cells, it is more common to use a transfection reagent in the culture medium rather than directly transferring the gene. This is because using a transfection reagent, such as TransFectin™, can significantly improve the transfection efficiency of Neuro-2a cells, resulting in higher rates of successful gene transfer. Direct transfer of genes into Neuro-2a cells without a transfection reagent can lead to lower transfection efficiency, making it more difficult to achieve successful gene transfer. Using a transfection reagent optimizes the transfection conditions, including factors such as pH, which can greatly enhance the efficiency of gene transfer into Neuro-2a cells. Additionally, using a transfection reagent-based system does not cause detectable cytotoxicity to the cells, making it a safe and effective method for gene transfection in Neuro-2a cells.
Are there NMDA receptors on GABA neurons?3 answers