Search or ask a question

Does the genetic modification of the OsSPL16 gene increase the yield of rice?

Pyruvate dehydrogenase complex

Pyruvate kinase

Pyruvate metabolic process

Best insight from top research papers

Genetic modification of the OsSPL16 gene through CRISPR/Cas9 technology has been shown to significantly increase the yield of rice. Mutants generated with OsSPL16 mutations exhibited a notable increase in grain yield without affecting other agronomic traits, as evidenced by proteomic analysis . Similarly, the OsSPL4 gene has been identified as a key regulator of grain size and panicle architecture, impacting grain weight and number, ultimately leading to enhanced rice yield . Additionally, OsSPL9 has been found to affect grain number per panicle and overall grain yield in rice, with favorable haplotypes contributing to high grain number identified . These findings collectively suggest that genetic modifications targeting OsSPL genes, including OsSPL16, can indeed boost rice yield significantly.

Answers from top 4 papers

PDF

Open Access

More filters

Papers (4)	Insight
Book Chapter•DOI Genomics, Biotechnology and Plant Breeding for the Improvement of Rice Production Kshirod K Jena, Sung-Ryul Kim - Show less +1 more 01 Jan 2020 3 Citations	Not addressed in the paper.
Open access•Journal Article•DOI OsSPL9 Regulates Grain Number and Grain Yield in Rice. Li Hu, Weilan Chen, Wen Yang, Xiaoling Li, Cheng Zhang, Xiaoyu Zhang, Ling Zheng, Xiaobo Zhu, Junjie Yin, Peng Qin, Yuping Wang, Bingtian Ma, Shigui Li, Hua Yuan, Bin Tu - Show less +14 more 02 Jun 2021-Frontiers in Plant Science 9 Citations	Not addressed in the paper.
Open access•Journal Article•DOI Programmed editing of rice (Oryza sativa l.) osspl16 gene using crispr/cas9 improves grain yield by modulating the expression of pyruvate enzymes and cell cycle proteins Babar Usman, Gul Nawaz, Neng Zhao, Shanyue Liao, Baoxiang Qin, Fang Liu, Yaoguang Liu, Rongbai Li - Show less +7 more 29 Dec 2020-International Journal of Molecular Sciences 34 Citations	Yes, programmed editing of the rice OsSPL16 gene using CRISPR/Cas9 increases grain yield by modulating pyruvate enzymes and cell cycle proteins, as shown in the study.
Open access•Journal Article•DOI The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice. Jihong Hu, Liyu Huang, Guanglong Chen, Hui Liu, Yesheng Zhang, Ru Zhang, Shilai Zhang, Jintao Liu, Qingyi Hu, Fengyi Hu, Wen Wang, Wen Wang, Yi Ding - Show less +12 more 02 Nov 2021-Rice 21 Citations	Not addressed in the paper.

My columns

Related Questions

Down-regulation of OsSPX1 causes high sensitivity to cold and oxidative stresses in rice seedlings4 answersDown-regulation of OsSPX1 in rice seedlings leads to increased sensitivity to cold and oxidative stresses. Similarly, the knockout of OsPRP1 also induces cold sensitivity in rice, with mutant plants displaying higher sensitivity to low temperatures and reduced antioxidant enzyme activity. Cold stress reduces bispyribac-sodium selectivity in rice, affecting lipid peroxidation and total phenol accumulation, while also influencing the expression of genes related to antioxidant enzymes and herbicide metabolism. Additionally, variations in the OsGSTZ2 gene in rice cultivars impact chilling sensitivity, highlighting the role of different isoforms in the response to chilling stress. Therefore, OsSPX1 down-regulation exacerbates cold and oxidative stress sensitivity in rice seedlings, emphasizing the intricate network of genes and pathways involved in stress responses.

What factors affects rice production in the philippines?5 answersFactors that affect rice production in the Philippines include various influencing factors such as demographic profile, constraints in rice farming, extension agents' role, other sources of income, monthly income in farming, and farm size. Additionally, labor, credit to agriculture, irrigated areas, and fertilizer have been found to significantly affect rice production. The adoption of direct-seeded rice (DSR) as an alternative to transplanted rice (TPR) has also been shown to impact rice production, with lower labor use and cost in crop establishment and higher labor productivity as benefits. Furthermore, enhancing access to irrigation and farm inputs, as well as increasing technical capacity, can potentially increase rice farm efficiency and productivity. It is important for policy interventions to consider the geographical location of farms to ensure the greatest contribution to attaining food production targets.

How recombinant DNA technology improve crop yields?4 answersRecombinant DNA technology improves crop yields by enhancing resistance to biotic and abiotic stresses, increasing nutrient uptake, and promoting plant growth. This technology allows for the insertion of selected traits into the plant genome, resulting in genetically modified crops with improved resistance and higher yields. It also enables the production of genetically modified crops with enhanced nutritional content, leading to improved food security. Additionally, recombinant DNA technology can be used to develop biofertilizers, which help maintain soil fertility and nutrient richness, leading to enhanced crop production. The use of transgenic technologies, such as insect-resistant endotoxins and genetic engineering techniques like RNAi and Crispr-Cas9, has also contributed to increased crop yields by providing effective pest management strategies. Overall, recombinant DNA technology plays a vital role in improving crop yields and addressing the challenges of food scarcity and agricultural sustainability.

What is the role of the D14 gene in rice plant height?4 answersThe D14 gene in rice plays a role in inhibiting shoot branching and controlling plant height. The d14 mutant exhibits increased shoot branching and reduced plant height, similar to other strigolactone-deficient and -insensitive mutants. However, unlike other mutants, the d14 branching phenotype cannot be rescued by exogenous strigolactones. D14 encodes a protein of the alpha/beta-fold hydrolase superfamily and functions downstream of strigolactone synthesis. It may act as a component of hormone signaling or as an enzyme involved in the conversion of strigolactones to the bioactive form. The role of D14 in rice plant height regulation is part of a complex regulatory network involving the biosynthesis and signal transduction of phytohormones such as gibberellins, brassinosteroids, and strigolactones.

Why grain yield increase due to stomatal conductance is low compared to other factors in rice?5 answersThe increase in grain yield due to stomatal conductance is relatively low compared to other factors in rice. Several studies have shown that factors such as plant height, lodging, leaf death response to heat, and canopy temperature depression (CTD) have a greater impact on grain yield than stomatal conductance. Additionally, the DEP1 gene, which affects the erect panicle architecture and increases grain yield, has been found to have a pleiotropic effect on photosynthesis and stomatal conductance under low nitrogen conditions. Furthermore, the manipulation of stomatal density in rice plants has been shown to improve drought tolerance and water use efficiency, leading to equivalent or improved yields despite a reduced rate of photosynthesis. These findings suggest that while stomatal conductance plays a role in grain yield, other factors such as plant architecture, nitrogen availability, and water use efficiency have a greater influence.

What is the background of genetic modification of crops?4 answersGenetic modification of crops involves the use of biotechnological techniques to alter the DNA of plants, introducing new characteristics that do not naturally occur in the species. This technology has been widely adopted by farmers, with significant increases in the acreage of genetically modified crops over the years. The goal is to improve crop traits such as herbicide tolerance and insect resistance, leading to higher yields and reduced production costs. However, genetic engineering still faces challenges in terms of technical limitations and the need for environmental and consumer safety. The development of genetically modified crops has the potential to address issues such as plant pathogens and yield losses, making it an important tool for sustainable agriculture. Despite the successes and prospects, there is a need for careful consideration of the potential impacts on human health and the environment.

See what other people are reading

What is the link between SLC16A2 and protein synthesis?

The SLC16A family, including SLC16A2, plays a crucial role in cellular metabolism and transport mechanisms that indirectly influence protein synthesis. SLC16A2 encodes the monocarboxylate transporter 8 (MCT8), primarily known for its specific role in thyroid hormone (TH) transport, which is essential for normal brain development and metabolic regulation. Thyroid hormones are critical regulators of gene expression, metabolic processes, and, by extension, protein synthesis. They influence the transcription of genes involved in the metabolic pathway, thereby affecting the availability of amino acids, the building blocks of proteins. Moreover, the SLC16A family members, such as SLC16A1, are involved in the transport of monocarboxylates like lactate and pyruvate across the plasma membrane, which are key metabolites in cellular energy metabolism. The energy status of a cell, regulated by monocarboxylate transporters, is fundamental to protein synthesis, as the process requires significant energy input. For instance, SLC16A1's role in tumor metabolism and its association with cancer progression and metastasis indirectly highlights the importance of energy metabolism in supporting the biosynthetic demands of rapidly proliferating cells, including protein synthesis. Furthermore, the interaction of SLC16A family transporters with other cellular mechanisms, such as pH regulation and fluid transport, underscores their contribution to creating an optimal intracellular environment for various biochemical processes, including protein synthesis. The regulation of intracellular pH is particularly important for maintaining enzyme activities that are crucial for protein synthesis. In summary, while SLC16A2's direct link to protein synthesis is not explicitly detailed in the provided contexts, its role in TH transport and the broader involvement of the SLC16A family in metabolic regulation and cellular homeostasis suggest an indirect but significant influence on protein synthesis through the regulation of metabolic pathways and maintenance of cellular energy status.What is the most commonly researched genetic mutations in humans?

The most commonly researched genetic mutations in humans encompass a wide array of pathogenic variations. These include single base-pair substitutions in coding, regulatory, and splicing-relevant regions of genes, micro-deletions, micro-insertions, gross insertions, duplications, repeat expansions, combined micro-insertions/deletions, gross deletions, inversions, and other complex rearrangements. Additionally, mutations introducing premature termination codons (X-SNPs) are of biological significance, with some causing transcript degradation and severe truncation of proteins. The study of mutations in human genes is crucial for understanding inherited disorders, optimizing diagnostic testing, and guiding therapeutic design. As genetic research progresses, the focus is shifting towards identifying mutations associated with mild phenotypes and exploring therapeutic approaches like pharmacological chaperones for specific mutations. Overall, a comprehensive understanding of various genetic mutations is essential for advancing molecular medicine and personalized healthcare.Can suberinase be used for protoplasting?

Suberinase, an enzyme involved in suberin degradation, has been studied in various organisms like Streptomyces scabiei. Protoplasting, a technique involving the enzymatic digestion of cell walls to produce protoplasts, has been explored in different organisms using enzymes like lysozyme and N-acetylmuramidase. While suberinase itself may not be commonly used for protoplasting, enzymes like Novozym 234 have been traditionally employed for this purpose in fungi like Aspergillus nidulans. The optimization of protoplasting conditions, such as enzyme concentrations and osmotic pressure, is crucial for successful protoplast formation and regeneration. Therefore, although suberinase may not be a typical enzyme used for protoplasting, understanding its role in cell wall degradation can contribute to the development of efficient protoplasting techniques in various organisms.What are popular genetic mutations for research papers?

Popular genetic mutations frequently studied in research papers include those related to hemoglobin disorders in Mediterranean and Muslim populations, as highlighted in a systematic analysis of mutation-related literature prevalence. Additionally, mutation analysis techniques have been extensively researched for over 40 years, with a focus on mutant selection optimization, mutant execution optimization, and applications such as test suite adequacy evaluation, test case generation, and regression testing. Furthermore, studies on mutation frequencies at specific loci in CHO cell lines after exposure to ionizing radiation have provided insights into the mutational events induced by X-rays, indicating the presence of essential sequences close to target genes and the potential for qualitative and quantitative analysis of mutagenic agents.What is the impact of salinity on rice seedling growth and development during the early stages?

The impact of salinity on rice seedling growth and development during the early stages is multifaceted, affecting various physiological and biochemical processes. Salinity stress has been shown to significantly reduce germination rates, seedling growth parameters, and alter mineral ion content in rice. For instance, increasing salinity levels were found to decrease germination rates and early seedling growth characteristics, with a notable reduction in root and shoot length, as well as in the vitality index of rice seedlings. Similarly, open field experiments revealed that salinity stress, especially during the early growth stages, led to stunted plant growth, reduced tillering, and compromised yield and quality by primarily affecting nitrogen uptake. Further studies on coastal rice landraces under varying salinity levels demonstrated differential responses among genotypes, with some showing lower reductions in shoot length, fresh and dry weight, and better maintenance of relative water content and electrolyte leakage, indicating varietal differences in salt tolerance. The application of biostimulants like humic acid has been found to ameliorate the negative effects of salinity by enhancing growth, maintaining ionic homeostasis, and improving antioxidant defense systems in rice seedlings. Additionally, the combined stress of drought and salinity further complicates the response, necessitating the development of screening protocols to evaluate rice cultivars under simultaneous stress conditions. Hydroponic studies have also highlighted the reduction in seedling parameters under saline conditions, with variations in Na+ and K+ ion concentrations and increased electrolyte leakage, indicating the physiological impact of salinity at the seedling stage. The ability to maintain a lower Na+/K+ ratio and higher activities of antioxidant enzymes under salinity stress was associated with better tolerance in certain rice genotypes. Screening of rice landraces for salinity tolerance at the germination stage revealed genotypic variations, with some landraces showing less reduction in growth parameters under high salinity levels. Transcriptomic analyses have identified differentially expressed genes related to protein kinase, calcium-binding, hormone signaling, and ion transport, which are activated during the early stages of salt stress, suggesting molecular mechanisms of response and adaptation. Lastly, studies on boro rice varieties under different salinity levels showed a wide variation in germination parameters and shoot and root growth, indicating the genetic basis of salinity tolerance. Collectively, these findings underscore the complex impact of salinity on rice seedling growth and development, highlighting the importance of genetic selection and management practices to mitigate the adverse effects of salinity stress.What is the role of cellular prion protein in phosphatidylinositol metabolism?

Cellular prion protein (PrPC) plays a significant role in lipid metabolism. It is a glycosylphosphatidylinositol-anchored protein primarily found in the plasma membrane, influencing various functions like neuroprotection and oxidative stress. PrPC's involvement in lipid metabolism is evident from studies showing excessive fat accumulation in the liver of PrPC knockout mice, along with altered expression of genes related to lipid metabolism. Additionally, over-expression of PrPC in hepatic cells led to a reduction in intracellular fat accumulation, further linking PrPC levels to lipid metabolism. Furthermore, PrPC has been associated with regulating glucose transporter 1 (Glut1) expression through the Fyn-HIF-2α pathway, impacting glycolysis and tumor growth in colorectal cancer cells.What Antifungal activity of chitosan against various pathogens fungi?

Chitosan exhibits significant antifungal activity against various pathogenic fungi. Studies have shown that chitosan, derived from chitin, possesses strong antifungal properties. Different forms of chitosan, including low, medium, and high molecular weight variants, as well as chitosan synthesized from crab shells, have been evaluated for their efficacy against fungal pathogens like Aspergillus fumigatus and Magnaporthe oryzae. The antifungal effects of chitosan are influenced by factors such as molecular weight, degree of deacetylation, and concentration, with higher degrees of deacetylation leading to increased inhibition of fungal growth. Additionally, combining chitosan with biocontrol agents like Debaryomyces hansenii F9D enhances its antifungal activity, making it a promising alternative to chemical fungicides for controlling fungal pathogens in various settings.How to make sure a mutation is homozygous in plant?

To confirm a homozygous mutation in plants, various methods can be employed. One approach involves utilizing high-throughput sequencing (HTS) combined with bulk segregant analysis after back-crossing to the parental line, which reduces genetic complexity and avoids issues with phenotype penetrance in different ecotypes. Another method includes screening and identifying homozygous mutants using the CRISPR/Cas9 system, where specific primers and PCR techniques are utilized to detect mutations in the mutant DNA compared to the wild type. Additionally, for induced mutations, thorough evaluation at the phenotypic level is crucial, as commonly practiced in conventional plant breeding, where mutations induced by various methods have been widely accepted and utilized in breeding programs. These combined approaches ensure accurate identification and confirmation of homozygous mutations in plants.How glutaminolysis is linked to glycolisys?

Glutaminolysis and glycolysis are interconnected metabolic pathways crucial for cancer cell proliferation and survival. Glutaminolysis provides biosynthetic precursors, while glycolysis meets the energy demands of rapidly proliferating cells. In cancer cells, the coordination between glucose and glutamine consumption is mediated by a CtBP-SIRT4-GDH axis, where CtBP represses SIRT4 expression to promote glutaminolysis, especially under high glucose conditions. Additionally, in hepatocellular carcinoma (HCC), the glutaminolysis pathway shifts between GOT1 and GDH1 depending on glucose availability, highlighting the metabolic flexibility of cancer cells. Furthermore, androgens regulate glutaminolysis in prostate cancer cells, with glutaminase inhibition affecting cell viability and migration, particularly in castrate-resistant prostate cancer (CRPC) cells. These findings underscore the intricate relationship between glutaminolysis and glycolysis in cancer metabolism.What is the mechanism of hemophilia A?

Hemophilia A is primarily caused by mutations in the factor VIII (FVIII) gene, leading to a deficiency or dysfunction of this coagulation protein. The severity of hemophilia A varies based on residual FVIII activity, classified as severe, moderate, or mild. In some cases, symptomatic carriers of hemophilia A exhibit severe manifestations due to skewed X-chromosome inactivation, where a pathogenic mutation on the non-hemophilic X chromosome leads to extreme inactivation. Additionally, genetic mechanisms such as tandem inversion duplications and complex genetic rearrangements within the F8 gene can contribute to the development of hemophilia A. Mathematical models have identified factors like low factor V levels as potential enhancers of thrombin generation in hemophilia A, shedding light on the intricate biological networks involved in the disease.Where KRT10 mutation work?

KRT10 mutations are implicated in various skin disorders. Specifically, KRT10 mutations have been identified in epidermolytic ichthyosis (EI) cases, where a missense mutation at position c.467G>A (p.Arg156His) in KRT10 was found in affected individuals. Moreover, hotspot mutations at the Arg156 position of KRT10 have been linked to epidermolytic acanthoma (EA). These mutations in KRT10 lead to alterations in the encoded protein, affecting its function and contributing to the pathogenesis of skin conditions like EI and EA. Therefore, KRT10 mutations play a crucial role in the development of these dermatological disorders, highlighting the significance of understanding the genetic basis of skin diseases for accurate diagnosis and potential therapeutic interventions.