Hoechst 33342 good for cell viability study?
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Hoechst 33342 can be a valuable tool for cell viability studies, but its efficacy depends on the concentration used and the specific application. Research indicates that lower concentrations of Hoechst 33342 (7-28 nM) are non-cytotoxic and do not impact cell viability, making it suitable for live cell imaging studies . Additionally, Hoechst 33342 has been shown to be effective in evaluating sperm viability through flow cytometry, especially when combined with propidium iodide (PI) for dual staining . However, caution is advised as higher concentrations of Hoechst 33342 can inhibit proliferation and impair cell viability, emphasizing the importance of optimizing exposure parameters for accurate results . Overall, when used appropriately, Hoechst 33342 can provide valuable insights into cell viability and function in various research settings.
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| Papers (4) | Insight |
|---|---|
| Hoechst 33342 at concentrations between 7-28 nM is suitable for cell viability studies as it does not affect cell proliferation, viability, or signaling pathways, allowing real-time high-throughput live-cell imaging. | |
| Yes, Hoechst 33342 is suitable for cell viability studies as it does not exhibit toxicity over a 6-hour period and provides consistent results with end-point assays. | |
15 Citations | Hoechst 33342 is relatively unsuitable for cell viability studies due to rapid redistribution into non-labeled cells, limiting its effectiveness as a cell marker in transplantation research. |
23 Citations | Yes, Hoechst 33342 (H342) is effective for assessing rhesus monkey sperm viability, showing strong correlation with expected viability and providing accurate results for flow cytometric analysis. |
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