How are antioxidants tested in the body?
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15 Dec 2017 11 Citations | Abundant evidence suggests that antioxidants play a pivotal role in the |
22 Citations | In addition, the developed method has potential application in the screening of antioxidants. |
20 Citations | This method offers the first means for comprehensively profiling the fate of antioxidants in tissue samples. |
01 Nov 1999 | In addition, the accumulation of antioxidants in selective tissues may not be apparent from plasma measurements. |
| Therefore, it is imperative to include antioxidants in our diets. | |
| The medicinal plants in this study tested are expected to be good sources of natural antioxidants. | |
| These results provide avenues for the therapeutic potential of antioxidants in a variety of diseases. |
Related Questions
How do antioxidants work in the body?5 answersAntioxidants function in the body by neutralizing free radicals, which are unstable molecules causing oxidative damage to lipids, proteins, and DNA. These free radicals are by-products of various metabolic processes and can lead to diseases like cancer, Alzheimer's, and diabetes. Antioxidants scavenge and remove free radicals, preventing oxidative stress and cellular damage. The body's antioxidant system, including vitamins like C and E, carotenoids, polyphenols, and minerals such as zinc and selenium, plays a crucial role in maintaining cellular health. While some antioxidants can act as pro-oxidants in specific conditions, a balance is essential for optimal health. Consuming antioxidant-rich foods like fruits, vegetables, and teas is recommended to counteract oxidative damage and promote overall well-being.
How can i do antioxidants test for oils?5 answersTo test the antioxidant content in oils, several methods can be used. One method involves using a mixed solution of sulfuric acid, ethyl alcohol, and silicon dioxide as a test reagent. This reagent is mixed with the oil sample and subjected to a linear voltammetry test to obtain a voltage-current curve. The phenolic antioxidants in the oil can then be quantified using an external standard method. Another method involves using constant-current coulometry with electrogenerated titrants. This method has been successfully applied to evaluate the antioxidant properties of essential oils. The reactivity of individual antioxidants in the oils is estimated by their reaction with electrogenerated bromine and ferricyanide ions. Total antioxidant capacity and ferric reducing power can be evaluated based on these reactions. Microcalorimetry can also be used to determine the antioxidant activity of oils. This method has been used to identify natural objects with high antioxidant activity for potential use as antioxidant additives in oils and other products. Additionally, simple electrochemical measurements in a bicontinuous microemulsion have been used to quantitatively analyze lipophilic antioxidants in oils. This method allows for the direct monitoring of antioxidants without the need for extraction. Finally, a screening method involving thin-layer chromatography and mass spectrometry has been developed to detect and quantify phenolic antioxidants in edible oils. This method offers accurate quantification and conclusive identification of multiple phenolic antioxidants.
How to test mitochondria?3 answersMitochondria can be tested using various methods. One common approach is the Mito Stress Test, which involves sequential exposure of cells to ATP synthase inhibitors and uncouplers to assess mitochondrial bioenergetic function. Another method is flow cytometry, which combines mitochondria-specific dyes or dyes targeting superoxide with surface marker staining to measure mitochondrial content and activity in live cells. Laser Induced Fluorescence (LIF) and Photoacoustic Spectroscopy (PAS) can be used to record fluorescence and photoacoustic signals of mitochondrial fractions, providing insights into mitochondrial dynamics. Live-cell fluorescent microscopy using dyes such as MitoTracker Green and LysoTracker Red allows for the evaluation of mitophagy, mitochondrial morphology, and quantification of mitochondria and lysosomes. Additionally, cell-permeable dyes like MitoTracker Green FM, MitoTracker Red CMXRos, tetramethylrhodamine methyl ester, and MitoSOX Red can be used to monitor mitochondrial volume, membrane potential, and the production of mitochondrial superoxide anion.
Why is there a need to compare antioxidant?5 answersComparing antioxidants is necessary for several reasons. Firstly, it allows for the identification of compounds with the highest antioxidant activity, which can be useful in the development of effective antioxidants. Additionally, comparing antioxidants helps in understanding their mechanism of action and their effectiveness in different biological systems. It also helps in evaluating the stability of the corresponding radicals and their ability to protect cells from oxidative damage. Furthermore, comparing antioxidants can provide insights into their role in preventing degenerative diseases and their bioavailability in the body. Standardized methods for comparing antioxidants are important for the food, nutraceuticals, and dietary supplement industries. Overall, comparing antioxidants allows for the selection of the most potent compounds, understanding their biological activity, and developing standardized methods for their evaluation.
What is the rationale for using this assay to assess antioxidant activity?5 answersThe rationale for using assays to assess antioxidant activity is to determine the ability of substances to prevent or delay oxidation and the formation of free radicals. Antioxidants play a crucial role in maintaining cellular health and preventing the development of diseases caused by oxidative stress. Various methods have been developed to measure antioxidant capacity, including electron transfer-based assays, hydrogen atom transfer-based assays, and lipid peroxidation assays. These assays provide valuable information about the antioxidant potential of molecules or extracts and can be used to evaluate their efficacy in scavenging free radicals and protecting cellular structures. Additionally, assays such as the Vesicle Conjugated Autoxidizable Triene (VesiCAT) assay offer insights into the distribution and interaction of antioxidants with reactive oxygen species in membrane models. By using multiple assays with different mechanisms, the full capability of an antioxidant sample can be revealed.
What are the main methods of measuring oxidative stress?5 answersThe main methods of measuring oxidative stress include measuring free radicals and active species by flow cytometry, determining the total antioxidant capacity of body fluids, measuring the oxidation products of macromolecules, determining the activity of antioxidant enzymes, and changing the expression of genes related to the antioxidant system. Another method involves inducing all blood cells to produce excessive reactive oxygen species (ROS) and measuring the fluorescence or color intensity of a dye that reacts with ROS. Additionally, a method for quantitatively measuring oxidative stress involves quantifying the level of oxidative stress using an influence factor. Other techniques include using specific biomarkers and probes to detect reactive species in cells and measuring oxidative species through lipid peroxidation, protein oxidation, and activity of antioxidant enzymes. Furthermore, a means for measuring reactive oxygen in the human body involves using a color indicator that undergoes a color change upon reaction with malondialdehyde, allowing for the quantitative detection of reactive oxygen.