How are recombinants obtained during protoplast fusion?
Best insight from top research papers
Recombinants are obtained during protoplast fusion through the fusion of protoplasts from different organisms. Protoplasts are induced by treating bacterial cells with lysozyme, and then regenerated on specific media . The fusion of protoplasts is achieved by combining the parental protoplasts in the presence of polyethylene glycol (PEG) . The resulting fusants are a genetically heterogeneous population with recombined mutations within the fused protoplasts . The frequency of recombination in fused protoplasts can vary, but in some cases, it has been reported to be as high as 0.7% . Protoplast fusion techniques have been successfully used to generate hybrids with different taxonomic characters and antibiotic activities . These techniques have shown great potential for strain improvement and the production of desired industrial properties .
Answers from top 5 papers
More filters
| Papers (5) | Insight |
|---|---|
4 Citations | The paper does not provide information on how recombinants are obtained during protoplast fusion. |
21 Citations | The paper does not provide information on how the recombinants are obtained during protoplast fusion. |
45 Citations | The paper states that recombinants are obtained during protoplast fusion in Escherichia coli at frequencies of 0.05-0.7% based on the number of protoplasts subjected to fusion. |
Open access 01 Jan 2010 9 Citations | Copilot couldn't generate the response. Please try again after some time. |
| The paper does not provide information on how recombinants are obtained during protoplast fusion. |
Related Questions
Why should i use recombinant DNA technology in the production of enzymes?5 answersRecombinant DNA technology should be utilized in enzyme production due to its ability to overcome limitations like low yields and reproducibility. This technology enables the high-level production of redesigned enzymes with improved properties tailored to specific industrial needs, enhancing performance in various sectors such as food, pharmaceuticals, and more. By cloning and expressing desired enzyme genes in host microorganisms, genetic engineering tools facilitate the production of high-yield enzymes suitable for modern food-processing conditions, leading to increased enzyme yields and reduced secondary metabolite production. Moreover, recombinant DNA technology has revolutionized enzyme production by allowing for the overproduction of industrially important microbial enzymes, contributing significantly to the enzyme market's recombinant origin.
What is recombinant mycobacteria? how is it made?5 answersRecombinant Mycobacteria (rMyc) are genetically modified mycobacteria that express specific proteins or fusion proteins. These modified bacteria can be used for various purposes, such as antigen production and diagnostic applications. The production of rMyc involves incorporating sequences encoding desired proteins into the mycobacterial genome, often under the control of specific promoters. For example, fusion proteins containing mycobacterial antigens can be produced abundantly by rMyc, post-translationally modified, and exhibit high antigenicity. Additionally, rMyc can mimic the diagnostic profiles of pathogens like Mycobacterium tuberculosis and Staphylococcus aureus, serving as safe and reliable quality controls for pathogen detection assays. The creation of rMyc involves advanced molecular techniques like PCR and specific antigen epitopes to enhance specificity and sensitivity in diagnostic kits.
What is the Significance of Recombinant DNA technology?4 answersRecombinant DNA technology has significant importance in various fields such as healthcare, agriculture, and environmental sustainability. In healthcare, it is used for the development of biopharmaceuticals, vaccines, gene therapy, and molecular diagnostics. In agriculture, recombinant DNA technology plays a crucial role in nutritional enhancement, crop yield improvement, and the production of biofertilizers. It allows for the insertion of specific traits into plant genomes, resulting in genetically modified plants with desired characteristics such as sustainable growth, enhanced yield, and increased tolerance to biotic and abiotic stresses. Additionally, recombinant DNA technology enables the production of genetically engineered microbial insecticides, which have advantages such as higher efficacy, lower resistance, and reduced chemical usage. Overall, the significance of recombinant DNA technology lies in its ability to address various challenges in healthcare, agriculture, and environmental conservation, leading to improved outcomes in these fields.
What is Recombinant DNA technology?5 answersRecombinant DNA technology is a strategic approach that involves the precise regulation of target gene expression for therapeutic purposes. It utilizes gene products to produce safe, cost-effective, and scalable therapeutics for the treatment and prevention of various diseases. This technology, also known as metabolic engineering of microbes, creates genetically modified organisms (GMOs) to produce desired products by interfering or modulating metabolic pathways. Molecular biology techniques, such as cloning and recombinant DNA techniques, are used to manipulate DNA sequences in vitro. These techniques involve cutting, ligating, and mass-producing DNA sequences to create useful DNA constructs for future experiments. Recombinant DNA technology has revolutionized the field of biotechnology by allowing scientists to edit the genetic makeup of organisms. It involves the use of enzymes for cutting, polymerization, and ligation, vectors as vehicles for DNA transfer, and host organisms as production factories. Overall, recombinant DNA technology is a crucial area of life engineering that explores the possibilities of repairing damaged human DNA and developing new treatments.
What is recombinant DNA technology?3 answersRecombinant DNA technology is a strategic approach that involves the precise regulation of target gene expression for therapeutic purposes. It encompasses the manipulation of DNA sequences in vitro, including the identification and cutting of specific DNA fragments from the genome, the insertion of these fragments into storage vectors, and the mass production of DNA constructs using bacteria. This technology allows for the creation of transgenic/genetically modified organisms (GMOs) by introducing desired genes into bacterial or yeast model systems, resulting in organisms with modified genetic constituents. Recombinant DNA technology has a wide range of applications, including the production of therapeutics, such as insulin, through the safe and scalable production of target proteins. It also plays a crucial role in gene therapy, genetic modification techniques, bioremediation, and the production of valuable metabolites in microorganisms. The technology relies on three main tools: enzymes for cutting, polymerization, and ligation; vectors as vehicles for DNA transfer; and host organisms as production factories.
What are the steps of the recombinant DNA technology process?4 answersRecombinant DNA technology involves several steps. First, a gene of interest is extracted using a restriction endonuclease enzyme and inserted into a self-replicating vector, such as a plasmid. The vector's DNA is cleaved using the same restriction endonuclease and sealed back using DNA ligase. The vector is then inserted into a host organism, such as E. coli or Saccharomyces cerevisiae, where it will multiply and be translated into proteins. Recombinant DNA technology has been used to synthesize hormones, vaccines, drugs, and genetically modified food. The process involves manipulating DNA in vitro using enzymes that cut and ligate DNA, as well as rearranging DNA in live cells through homologous or site-specific recombination. Cloning vectors and strategies are chosen based on experimental goals, and cloned DNA is replicated, amplified, and purified for further manipulations. Isolating DNA from bacteria, such as E. coli, is the easiest procedure due to the simplicity of bacterial cells.