How does pco2 impact charge variant in CHO cell cultures?
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Elevated pCO2 concentrations in CHO cell cultures have been found to impact charge variants. Studies have shown that at higher pCO2 levels, the lactate metabolic shift, which is important for cell culture longevity and final product titers, is absent compared to cultures at lower pCO2 values . Additionally, interactions between process parameters such as pH and pCO2 have been found to affect charge variants of monoclonal antibodies (mAbs) produced by CHO cells . Furthermore, the concentration of Zn2+, duration of culturing, and temperature have been shown to affect charge variants of mAbs, and the addition of carboxypeptidase B (CpB) can significantly improve the main peak of mAbs without affecting other critical quality attributes . These findings suggest that pCO2 and other process parameters can have a significant impact on charge variants in CHO cell cultures, highlighting the importance of understanding and controlling these parameters for optimizing mAb production.
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| The paper mentions that the process pCO2 has an impact on monoclonal antibody (mAb) charge variants in CHO cell cultures. However, the specific details of this impact are not provided in the paper. | |
| The provided paper does not mention anything about the impact of pCO2 on charge variants in CHO cell cultures. | |
33 Citations | The provided paper does not discuss the impact of pCO2 on charge variant in CHO cell cultures. |
15 Citations | The provided paper does not mention the impact of pCO2 on charge variants in CHO cell cultures. |
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