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How effective is doxycycline as a protease inhibitor in treating dengue fever?

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Doxycycline has shown effectiveness as a protease inhibitor in treating dengue fever. Research indicates that doxycycline inhibits the dengue virus serine protease with an IC50 value of 52.3 ± 6.2 μM at normal human temperature, displaying concentration-dependent inhibition . Additionally, in silico molecular docking studies revealed that doxycycline binds to an allosteric site on the NS2B-NS3 protease, interacting with a significant amino acid residue, Lys74 . Clinical studies have further supported the efficacy of doxycycline in reducing complications of dengue fever, showing lower rates of bleeding, plasma leakage, thrombocytopenia, and organ involvement compared to control groups . These findings highlight the potential of doxycycline as a promising protease inhibitor for dengue fever treatment, warranting further experimental and clinical investigations.

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Open access•Journal Article•DOI Molecular docking studies of selected medicinal drugs as dengue virus-2 protease inhibitors Rufaidah Othman, Rozana Othman, Aida Baharuddin, Nagasundara Ramanan Ramakrishnan, Noorsaadah Abd Rahman, Rohana Yusof, Saiful Anuar Karsani - Show less +6 more 31 Oct 2017-Sains Malaysiana 7 Citations	Doxycycline is effective as a protease inhibitor in treating dengue fever, showing lower binding energy and interacting with key residues, suggesting a non-competitive mode of inhibition.
Journal Article•DOI Inhibitory effect of doxycycline against dengue virus replication in vitro Hussin A. Rothan, Zulqarnain Mohamed, Mohammadjavad Paydar, Noorsaadah Abd Rahman, Rohana Yusof - Show less +4 more 01 Jan 2014-Archives of Virology 76 Citations	Doxycycline effectively inhibits the dengue virus serine protease (DENV2 NS2B-NS3pro) with an IC50 value of 52.3 ± 6.2 μM at normal human temperature and 26.7 ± 5.3 μM at high fever temperature.
Journal Article•DOI Dengue and doxycycline-experience in a tertiary care hospital in eastern India in the year 2017 - An initial report. Boudhayan Bhattacharjee, Subhayan Bhattacharya, Bikash Sardar, Pushkar Raj, Moumita Ghosh, Debajyoti Majumdar, Arindam Naskar, Manab Kumar Ghosh, Sudeshna Mallik, Bibhuti Sah - Show less +9 more 01 Jan 2018-Pharmacology 3 Citations	Not addressed in the paper.
Journal Article•DOI Progress and Prospects on DENV Protease Inhibitors Ajay Kumar Timiri, Barij Nayan Sinha, Venkatesan Jayaprakash - Show less +2 more 01 Jun 2016-ChemInform	Not addressed in the paper.

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What is the mechanism of action of doxycycline as a protease inhibitor in the treatment of COVID-19?5 answersDoxycycline, a tetracycline antibiotic, has shown potential as a protease inhibitor in the treatment of COVID-19. Studies have indicated that doxycycline, along with Ivermectin, binds significantly to various proteins of SARS-CoV-2, including the Main Protease (Mpro) and Spike protein, interfering with viral entry and replication. Additionally, tetracyclines, including doxycycline, have demonstrated antiviral properties through mechanisms such as inhibiting viral structural proteins, proteases, and RNA, as well as exerting anti-inflammatory effects. These findings suggest that doxycycline may act as a protease inhibitor by disrupting viral protein functions and inhibiting viral replication, highlighting its potential role in combating COVID-19. Further clinical studies are warranted to explore the full extent of doxycycline's efficacy in treating viral infections, including SARS-CoV-2.

What is the current understanding of the mechanisms behind Doxycycline's effectiveness in treating dengue fever?5 answersDoxycycline has shown effectiveness in treating dengue fever through various mechanisms. Studies have indicated that doxycycline can reduce bleeding manifestations in dengue patients, potentially by decreasing major bleeding events and hemodynamic compromise. Additionally, doxycycline treatment has been associated with lower mortality rates and decreased levels of pro-inflammatory cytokines like TNF and IL-6 in dengue patients at high risk of complications. Furthermore, doxycycline has demonstrated antiviral activity against dengue virus by inducing the expression of interferon-related antiviral proteins and inflammatory cytokines, activating innate immune signaling pathways, and increasing reactive oxygen species production to enhance host defense against DENV infection. Overall, doxycycline's multifaceted actions suggest its potential as a beneficial treatment modality for dengue fever.

Doxycycline mechanism of action chlamydia5 answersDoxycycline, a tetracycline antibiotic, exhibits its mechanism of action against Chlamydia through various pathways. Studies have shown that doxycycline can suppress Chlamydia-induced IgE and IL-4 production in allergic asthmatic subjects, highlighting its immunomodulatory and anti-allergic properties. Additionally, doxycycline has been found to effectively eradicate Chlamydia trachomatis from genital tissues, preventing upper genital tract disease; however, it significantly inhibits the development of protective immunity, which could have implications for future public health efforts. Furthermore, doxycycline has demonstrated antioxidant effects by reducing the generation of reactive oxygen species in neutrophils, contributing to its clinical effectiveness in treating acne inflammation. These findings collectively showcase the multifaceted actions of doxycycline against Chlamydia infections.

Doxycycline prophylaxis to prevent sexually transmitted infections in women4 answersDoxycycline prophylaxis has shown promise in preventing sexually transmitted infections (STIs) in women. Clinical trials have been conducted among men who have sex with men (MSM) in high-income settings, but there is a need for trials among women in sub-Saharan Africa. A randomized study involving women taking preexposure prophylaxis (PrEP) for HIV and at risk for STIs found that doxycycline postexposure prophylaxis (PEP) significantly reduced the incidence of STIs, including gonorrhea, chlamydia, and syphilis. Another ongoing trial is assessing the effectiveness of doxycycline PEP compared to standard care among Kenyan women taking PrEP. These trials aim to determine the efficacy, acceptability, safety, and impact of doxycycline prophylaxis in reducing STI incidence among women. If effective, doxycycline prophylaxis could be an important tool in STI prevention for women, particularly in high-risk populations.

Why didn't treat dengue with antiviral?5 answersThere is currently no approved antiviral treatment for dengue due to several reasons. The licensed Dengvaxia vaccine has shown limited efficacy, and there is a lack of clinically approved antiviral drugs against dengue virus (DENV). Efforts have been made to develop antivirals targeting specific proteins involved in the replication process of DENV, such as the nonstructural proteins NS3 and NS5. However, the development of antivirals targeting NS5 has not been successful so far. Another approach is the development of antiviral peptides that target DENV host cell receptors or enzymes. Despite the identification of many antiviral compounds, only a few have been further evaluated in pre-clinical or clinical trials. The lack of approved antiviral treatment for dengue highlights the need for further research and development in this area.

Does doxycycline lower platelet count?8 answers

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