How is agrobacterium tumefaciens used in gene editing?
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Agrobacterium tumefaciens is utilized in gene editing through various strategies. One approach involves the use of CRISPR RNA-guided integrase systems, like the INTEGRATE method, to facilitate targeted gene knockouts and precise deletions of DNA segments . Another method focuses on engineering the adhesion properties of A. tumefaciens by introducing genetically encoded exogenous adhesins, enhancing its ability to target host cells and improve DNA transfer efficiency, especially to non-natural hosts . Additionally, CRISPR-mediated base editing has been developed to introduce targeted point mutations into the genomes of Agrobacterium species, enabling the creation of more efficient strains for plant transformation and gene editing purposes . These diverse approaches highlight the versatility of Agrobacterium tumefaciens in gene editing applications.
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| Papers (5) | Insight |
|---|---|
| Agrobacterium tumefaciens is utilized in gene editing through a CRISPR RNA-guided integrase system called INTEGRATE, enabling efficient targeted gene knockouts and precise deletions of large DNA fragments. | |
| Agrobacterium tumefaciens is utilized in gene editing through CRISPR-mediated base editing, enabling efficient targeted point mutations in its genome for improved plant transformation and genetic engineering applications. | |
| Agrobacterium tumefaciens is engineered with exogeneous adhesins to enhance adhesion to target cells, improving DNA transfer efficiency for genetic modification purposes, including gene editing. | |
14 Jun 2022 | Agrobacterium tumefaciens is utilized for transferring T-DNA into plant genomes, enabling gene editing and expression analysis in plant genetic engineering and molecular biology studies. |
| Agrobacterium tumefaciens is engineered with exogeneous adhesins to enhance adhesion to target cells, improving DNA transfer efficiency for genetic modification purposes, including gene editing. |
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