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With this optimized synthetic procedure, relatively large collagen‐type peptides were obtained in satisfactory yields as highly homogeneous compounds.
The topics may serve as a guide for the design of collagen-like peptides and their bioconjugates for targeted application in the biomedical arena.
The methods outlined here provide a reliable means by which identifiable type IV collagen peptides can be isolated.
Collagen I prepared with these procedures is highly suitable for many in vitro applications.
The nature of the marker peptides, the use of high-performance liquid chromatography techniques, and quantitation of the peptides by ultraviolet absorbance renders the method suitably rapid, sensitive, and accurate for routine evaluations of collagen composition.