How to attach epitopes on vlp with Nhs and edc?
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To attach epitopes on Virus-Like Particles (VLPs) using NHS and EDC, epitopes can be genetically inserted into the VLP protein at different sites. Additionally, EDC-NHS-activated protein A can be utilized for site-directed immobilization of antibodies on a waveguide-coupled bimetallic Surface Plasmon Resonance (WcBiM SPR) sensor, showing increased sensitivity and lower detection capability compared to protein A and self-assembled monolayers. This method involves soaking the biological valve material in an EDC/NHS solution followed by cleaning, then soaking it in a recombinant human collagen solution to enhance its properties. By following these approaches, researchers can effectively attach epitopes on VLPs using NHS and EDC for various applications in immunology and sensor development.
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| Papers (4) | Insight |
|---|---|
| The paper outlines using broadly neutralizing antibodies (bNAbs) to capture VLPs displaying neutralization-sensitive epitopes, facilitating epitope attachment prediction for eliciting a neutralizing B cell response. | |
| The paper does not address the attachment of epitopes on VLPs with NHS and EDC. | |
1 Citations | The paper focuses on using broadly neutralizing antibodies (bNAbs) to capture VLPs displaying neutralization-sensitive epitopes, not on attaching epitopes with Nhs and edc. Not addressed in the paper. |
| The EDC-NHS method can be used to attach epitopes on VLP by activating the protein with EDC and NHS for site-directed immobilization, as demonstrated in the study. |
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