How to flush channels in hydrogel with endothelial cells?
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To flush channels in hydrogels with endothelial cells, various innovative techniques have been developed. One method involves creating hollow, perfusable channels by encapsulating polycaprolactone fibers in cell-laden hydrogels and physically removing them, allowing for the formation of interconnected grids or hierarchically branched patterns . Another approach includes fabricating biofunctionalized polyethylene glycol hydrogel microchannels with adjustable circular cross-sections, decorated with Au-nanoparticle arrays functionalized with biomolecules, enabling control over flow conditions and biomolecule density on the channel surface . Additionally, three-dimensional bio-printing technology has been utilized to engineer vascular structures within hydrogel scaffolds, where endothelial cells are seeded in tubular form within a collagen scaffold, creating fluidic channels connected to a perfusion system for stable flow conditions . These methods showcase advancements in creating perfusable channels in hydrogels with endothelial cells for various tissue engineering applications.
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| Papers (5) | Insight |
|---|---|
03 Apr 2009 5 Citations | Flushing channels in hydrogel with endothelial cells involves seeding cells into fabricated microchannels, allowing for nutrient exchange and waste removal, promoting cell viability and proliferation within the hydrogel construct. |
15 Citations | Endothelial cells can be flushed into hydrogel channels by perfusing TCO-conjugated molecules into the lumen, allowing spatial patterning of vascular cells for creating functional arteries. |
01 Apr 2011 5 Citations | To flush channels in hydrogel with endothelial cells, use a layer-by-layer approach with endothelial cells in heated gelatin, followed by gelatin liquefaction and washing out to create fluidic channels. |
31 Citations | Circular hydrogel channels with biofunctionalized nanostructures can be flushed with endothelial cells by controlling flow conditions and biomolecule density on the channel surface, mimicking natural vessel environments for cell adhesion studies. |
| Perfusable channels in hydrogels can be flushed with endothelial cells by creating hollow channels using melt-electrowritten fibers, enabling nutrient supply and potential applications in tissue engineering. |
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