How to make a calibration e.coli curve in uv/vis spectrophotometer?
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To create a calibration curve for E. coli in a UV/Vis spectrophotometer, a method involving chromogenic media preparation, E. coli culturing, microbial metabolism for color generation, and UV/Vis detection is employed . The calibration process optimizes the spectrophotometer's performance using a normal calibration method and Ringbom-Ayre's plot for precision confirmation . For E. coli biomass concentration measurement, UV-Vis spectroscopy with SNV and MSC methods is utilized, followed by two-dimensional correlation analysis to capture absorption characteristics and establish a prediction model using Extreme Learning Machine network . This method yields accurate results with a coefficient of determination (R2) of 0.92741 and significantly reduces analysis time, making it suitable for online measurements in dairy products .
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| The ultraviolet visible spectrophotometric method in the paper involves configuring chromogenic media, culturing E. coli, oxidizing a substrate to produce color, and quantitatively detecting E. coli, facilitating calibration curve creation. | |
27 Mar 2022 | The paper proposes using UV-Vis spectroscopy with SNV and MSC for E. coli biomass measurement in milk. It employs two-dimensional correlation analysis and an Extreme Learning Machine network for calibration. |
| Not addressed in the paper. |
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