How to make NBT solution for ROS complete method?
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To prepare a nitroblue tetrazolium (NBT) solution for reactive oxygen species (ROS) analysis, one can follow a method involving the electrochemistry of NBT. The NBT solution can be utilized for detecting superoxide, a type of ROS, by exploiting the sensitivity of diformazan oxidation signal . Additionally, a quantitative measurement method for total ROS in a solution involves creating a standard calibration curve using H2O2 and H2DCF-DA, measuring fluorescence values, and calculating H2O2 content based on the curve . Furthermore, a ROS-based programmable logic controller (PLC) system allows for message exchange between multiple PLCs, facilitating data acquisition and processing for ROS analysis . By integrating these methods, one can effectively prepare and analyze NBT solutions for comprehensive ROS detection and quantification.
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| The NBT solution for ROS can be prepared by immersing a carbon paste electrode in superoxide solution, followed by equilibration with NBTC in a phosphate buffer solution at pH 6.97. | |
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