Is living organisms dead in transmission electrons microscope?
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Living organisms can survive in transmission electron microscopy (TEM) under certain conditions. Some organisms, including bacteria, fungi, plants, and animals, have been reported to survive hostile scanning EM (SEM) conditions without chemical fixation or after coating with a thin polymer layer . However, the minimal needed electron dose to obtain contrast in TEM is many orders of magnitude above the lethal dose known to cause reproductive-cell death . To visualize living cells with TEM, a low-dose STEM technique has been developed, which allows for the imaging of virus infection in living cells while preserving cell viability . TEM can also be used to visualize morphological changes during programmed cell necrosis, providing a single-step methodology for characterization of cell-programmed necrosis based on morphology . Therefore, while living organisms can survive in certain conditions, the standard TEM technique may not be suitable for imaging live cells without compromising cell viability.
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| Papers (5) | Insight |
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39 Citations | The paper does not directly answer the question of whether living organisms are dead in transmission electron microscopy. The paper focuses on visualizing living cells using scanning transmission electron microscopy (STEM) without compromising cell viability. |
5 Citations | The provided paper does not directly address whether living organisms are dead in transmission electron microscopy. |
4 Citations | The provided paper does not mention anything about transmission electron microscopy or whether living organisms are dead in transmission electron microscopy. |
| The provided paper is about the survival of certain organisms during scanning electron microscopy (SEM) conditions. It does not mention transmission electron microscopy (TEM) or whether living organisms are dead in TEM. | |
73 Citations | Living organisms are not dead in transmission electron microscopy, but the minimal electron dose needed for contrast is many orders of magnitude above the lethal dose for reproductive-cell death. |
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