Is RNA and PCR test the same?
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| On the basis of the derived results we can argue that qRT-PCR performance is affected by the RNA integrity and PCR efficiency in general is not affected by the RNA integrity. | |
95 Citations | In addition, we showed that stored RNA is compatible for further analyses, such as reverse transcription-quantitative PCR. |
Open access•Journal Article 48 Citations | In our hands, RNA template-specific PCR retains the sensitivity of the traditional method, but greatly reduces the frequency of false positives, and virtually eliminates carryover contamination from PCR products amplified in previous experiments. |
| The results indicate that RNA was of good quality and fit for real time PCR. | |
60 Citations | As demonstrated in this study, the level of a particular RNA may be reliably determined by RT-PCR even in a total RNA that is usually considered as degraded (28S to 18S ratio as low as 0.4), provided that random primer is used in RT. |
65 Citations | The use of a synthetic RNA standard with identical sequences to the PCR primers allows reproducible quantitation between samples and assays. |
| Therefore, Db-PCR provides a much-needed simple method for analyzing RNA terminal heterogeneity. | |
62 Citations | The qualitative NASBA RNA assay is highly specific and more sensitive than DNA PCR. |
45 Citations | These results show that the method of RNA extraction we propose is suitable for giving accurate real time quantitative RT-PCR results. |
26 Citations | The one-step, real-time quantitative RT-PCR is a highly sensitive and specific, yet practically simple, RNA detection technique. |
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