Is RNA stable?
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RNA stability is a critical aspect influenced by factors like RNA decay rates, storage conditions, and interactions with ribonucleoprotein complexes. Various methods and reagents have been developed to stabilize RNA, such as using high temperatures and specific pH conditions with ammonium sulfate , or employing RNAstable® to store RNA in a dehydrated state at room temperature, protecting it from degradation . Additionally, RNA stability is linked to cellular processes like cell death, aging, and disease, emphasizing its importance in maintaining cellular RNA concentrations . Understanding RNA stability is crucial for preserving RNA integrity, which is essential for its functions in coding for proteins, structural roles, and regulatory functions within the cell.
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| Papers (5) | Insight |
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10 Citations | RNA stored in RNAstable® at room temperature showed similar quality to RNA stored at -80°C for extended periods, indicating RNA stability in RNAstable® as evaluated by expression microarray. |
| RNA can be stabilized at temperatures of at least 70 °C and pH levels of at least 8, preferably 9, with the presence of ammonium sulfate at concentrations of 10 mM or less. | |
13 Citations | RNA stability is crucial for cell function, as it codes for proteins and regulates processes. The paper discusses RNA stability in relation to cell death, aging, and disease correlations. |
| RNA stability varies genome-wide, influenced by splicing-related features positively correlating with stability, while miRNA binding and DNA methylation show negative correlations. Various histone modifications also impact RNA stability. | |
| RNA stability is enhanced by a hairpin RNA fragment containing specific sequences, inhibiting degradation and contributing to the stability of the RNA molecule described in the paper. |
Related Questions
How long is RNA stable for at RT blood collection?5 answersRNA stability at room temperature (RT) for blood collection varies based on the storage conditions and the type of blood collection tubes used. Studies have shown that RNA in stabilized blood tubes can remain stable for up to five days at RT. Additionally, long-term storage of blood samples in Tempus tubes at -80°C has demonstrated stability of RNA for up to six years. Another study highlighted that free RNA in blood collection tubes can be protected and stabilized for three days at RT. Furthermore, plasma cell-free RNA (cfRNA) stability was observed for up to 6 hours at RT or 4°C, with minimal variations in mRNA, lncRNA, and miRNA levels. Overall, RNA stability at RT for blood collection can range from a few days to several years, depending on the storage conditions and the specific blood collection tubes utilized in the study.
Is RNA stable in vitro?5 answersRNA stability in vitro varies depending on the specific conditions and factors involved. Studies have shown that RNA can maintain stability under certain circumstances. The stability of RNA duplexes in crowded environments has been investigated, with parameters determined for predicting stability accurately. Research on stress granules has highlighted the role of RNA helicases in regulating RNA-RNA interactions, impacting RNA condensate formation and stability. In contrast, microRNA has been found to be relatively stable compared to mRNA under defined ribonuclease treatment conditions, suggesting differential stabilities among RNA classes. Additionally, RNA folding stability within live mammalian cells has been compared to in vitro conditions, showing variable stability influenced by cellular effects. Overall, RNA stability in vitro is influenced by a range of factors, highlighting the complexity of RNA behavior in different environments.
How does cadmium exposure affect the stability of RNA-protein complexes?5 answersCadmium exposure impacts the stability of RNA-protein complexes through various mechanisms. Cadmium disrupts RNA processing, acting as a signal for environmental stress, leading to aberrant pre-mRNA splicing and induction of the heat shock response. Additionally, cadmium exposure can lead to changes in protein structures, affecting macromolecular complexes containing cadmium, such as metallothionein in oysters, which play a role in detoxifying harmful metals and shuttling them away from vital biological processes. Furthermore, cadmium's interaction with DNA repair mechanisms, cell cycle checkpoints, and epigenetic gene expression control can induce genomic instability, affecting the stability of RNA-protein complexes. These interactions highlight the intricate ways in which cadmium exposure can disrupt RNA-protein complexes and impact cellular functions.
How stable are circular RNA molecules?5 answersCircular RNA molecules are highly stable. Their closed ends contribute to their stability, although they can still be degraded by circulating endonucleases or microRNA-mediated cleavage. Circular RNAs have been shown to be more stable than their linear mRNA counterparts in vivo. They can accumulate to higher levels than linear mRNAs and are expressed in a tissue- and stage-specific manner. Circular RNAs have the ability to sponge microRNAs and regulate cellular processes. They can also act as competing endogenous RNAs and control gene expression events. Circular RNAs have been found to be abundant, stable, conserved, and nonrandom products of RNA splicing. Their stability, conservation, and regulatory functions suggest that they likely impact many biological processes.
What is the stability of lanreotide?3 answersLanreotide has been shown to have stability in various studies. In one study, lanreotide-loaded self nano-emulsifying delivery systems (SNEDS) demonstrated significant protection against thiol-disulfide exchange reactions. Another study found that lanreotide labeled with 131I using Chloramine-T as an oxidizing agent showed a radiolabeling yield of approximately 80%. Additionally, a prolonged-release formulation of lanreotide administered every 12 weeks was found to have a pharmacokinetic profile suggesting stability and a dosing interval of 12 weeks. In a retrospective study, lanreotide monotherapy was well tolerated and contributed to disease stabilization in patients with gastroenteropancreatic neuroendocrine tumors previously treated with octreotide. Finally, lanreotide labeled with 188Re demonstrated stability in vitro and biodistribution studies showed excretion primarily through the hepatobiliary system.
What is the most relevant review abour RNA stability for therapeutics?4 answersRNA stability for therapeutics is a topic that has been extensively reviewed in the literature. Several papers provide relevant information on this subject. Uchida et al. discuss the importance of balancing safety and stability in RNA delivery systems, highlighting the need for optimization for each RNA species. Zhu et al. comprehensively summarize the challenges and solutions for promoting RNA intracellular trafficking and metabolic stability in RNA-based therapeutics. Boada et al. focus on the mechanisms of action of RNA-based drugs and their potential for treating cardiovascular disease, with a discussion on recent advances in nanotechnology aiding in the translation of these therapies into the clinic. Fu et al. review the progress in RNA-based antitumor therapeutics, including stability and delivery of RNA drugs, as well as their mechanisms of inducing antitumor responses. Zhang et al. provide an overview of different classes of RNA-based therapeutics, including stability considerations, and discuss their potential in clinical trials.