Isolation, characterization, and transcriptome profiling of Pig (Sus scrofa) Umbilical cord mesenchymal stem cells
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Isolation and characterization of Pig Umbilical Cord Mesenchymal Stem Cells (pUC-MSCs) were successfully achieved in a study by Garcia et al.. The protocol involved rigorous disinfection procedures due to the non-controlled environment of farm pigs. The isolated pUC-MSCs maintained their cellular attributes and multipotential differentiation during in vitro proliferation. Additionally, studies on human umbilical cord-derived MSCs shed light on the heterogeneity and characteristics of cultured UMSCs at a single-cell resolution, providing insights into the transcriptomic differences between in vitro and in vivo subgroups. Furthermore, research on human UC-MSCs highlighted the importance of separating UC-MSCs into distinct populations based on gene expression profiles for tailored in vitro expansion and disease-specific treatments.
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