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ISSR markers based on profiling studies of Rauwolfia 


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ISSR markers have been used for profiling studies of Rauwolfia species. In one study, a protocol was developed for DNA profiling of five medicinally important Rauwolfia species using ISSR-PCR method. The study found that the HY3 primer produced distinct DNA bands ranging from 300 bp to 1500 bp, allowing for differentiation and identification of the species . Another study focused on Rauwolfia tetraphylla and used ISSR markers to assess the genetic fidelity of in vitro clonally multiplied plantlets. The study found that the regenerated plantlets showed no genetic variations compared to the mother plant, confirming their true-to-type nature . Additionally, ISSR markers have been used to evaluate the genetic diversity of Roegneria species, providing valuable information for forage breeding and germplasm collection . In another study, ISSR markers were used to analyze the genetic fidelity of regenerated Rauvolfia serpentina plants, with one marker showing a polymorphic band among the regenerants . Finally, ISSR markers have been used to characterize different taxa within the "Chirayat" complex, including various Swertia species, demonstrating the utility of ISSR markers for molecular authentication of medicinal plants .

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The paper discusses the development of a protocol for DNA profiling of Rauwolfia species using ISSR-PCR method. The HY3 primer was found to be effective in generating distinct DNA bands for identification of the species.
The paper discusses the use of 18 ISSR markers for genetic fidelity analysis of regenerated Rauvolfia serpentina plants, with one marker showing a polymorphic band among the callus regenerants.
The provided paper does not mention anything about ISSR markers based on profiling studies of Rauwolfia.
The paper mentions that ISSR markers were used to assess the genetic stability of micropropagated and acclimated plantlets of Rauwolfia tetraphylla.
The provided paper does not mention any studies on ISSR markers based on profiling of Rauwolfia.

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ISSR for genetic test in horticulture plants especially for abitik stress5 answersISSR (Inter-Simple Sequence Repeat) markers have been utilized in horticultural crops to assess genetic diversity, especially in plants facing abiotic stresses. These markers have been employed in grapevine rootstocks analysis, revealing genetic similarities and variability among different genotypes. Additionally, molecular markers, including ISSR, have been crucial in marker-assisted selection (MAS) for developing abiotic stress-tolerant horticultural varieties. The use of ISSR markers, along with SSR (Simple Sequence Repeat) markers, has enabled the identification of alleles and heterozygosity levels in grape genotypes, aiding in genetic diversity studies. Overall, ISSR markers play a significant role in understanding genetic relationships, variability, and selection of stress-tolerant traits in horticultural crops, contributing to the improvement of crop resilience to abiotic stresses.
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