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Optical density of Bacillus sibitulis?

Fourier transform infrared spectroscopy

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The optical density of Bacillus subtilis has been extensively studied in various contexts. Research has shown that the optical density of B. subtilis cultures can decline drastically due to mechanisms like cell lysis, cannibalism, and prophage-induced lysis . Studies utilizing Fourier transform infrared spectroscopy (FTIR) have calculated the mass extinction cross-section and complex refractive index of B. subtilis spores, providing insights into their absorption and scattering characteristics . Additionally, spectral reflectance and transmittance measurements have been used to determine the real and imaginary parts of the complex refractive index of B. subtilis spores, showing variations based on the spore's exposure history and suspension medium . These findings contribute to a deeper understanding of B. subtilis optical properties and behavior in different environments.

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Open access•Journal Article•DOI Incorporation of Optical Density into the Blending Design for a Biocement Solution M Fukue, Zbigniew Lechowicz, Yuichi Fujimori, Kenta Emori, Catherine N. Mulligan - Show less +4 more 01 Mar 2022-Materials 2 Citations	Not addressed in the paper.
Open access•Posted Content•DOI Drastic decline in optical density during stationary phase meant that Bacillus subtilis NRS-762 is not suitable as model organism in microbial survivability studies Wenfa Ng 13 Apr 2018	The optical density of Bacillus subtilis NRS-762 drastically declines during stationary phase, indicating cell lysis and making it unsuitable as a model organism for microbial survivability studies.
Open access•Journal Article Optical properties research of Bacillus subtilis spores by Fourier transform infrared spectroscopy Feng Mingchun, Liang Xu, Min-guang Gao, Yang Jiao, Xiu-li Wei, Ling Jin, Si-yang Cheng, Xiang-xian Li, Feng Shuxiang - Show less +8 more 01 Dec 2012-Spectroscopy and Spectral Analysis 7 Citations	The optical properties research of Bacillus subtilis spores included calculating mass extinction cross section and complex refractive index, but did not directly mention optical density.
Journal Article•DOI Optical properties of Bacillus subtilis spores from 0.2 to 2.5 num. P S Tuminello, E. T. Arakawa, B N Khare, J M Wrobel, Marvin R. Querry, M E Milham - Show less +5 more 01 May 1997-Applied Optics 77 Citations	The paper does not directly address the optical density of Bacillus subtilis.
Journal Article•DOI Correction for the inherent error in optical density readings. J V Lawrence, S Maier - Show less +1 more 01 Feb 1977-Applied and Environmental Microbiology 54 Citations	Not addressed in the paper.

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Related Questions

Why the optical density is 1 in spektrofotometer UV -Vis?5 answersThe optical density (OD) of 1 in a UV-Vis spectrophotometer is significant due to its role as a common metric for estimating chromophore formation and cell concentration in liquid cultures. The OD value of 1 represents a crucial point in the measurement scale, indicating a specific level of light attenuation primarily due to scattering, which is essential for accurate biomass concentration estimation. However, the accuracy of OD measurements is influenced by various factors such as the spectrophotometer model, wavelength used, and culture conditions, emphasizing the need for careful consideration when using OD as a proxy for direct measurements like dry weight, cell count, and pigment levels. Additionally, the relationship between OD and cell titer is complex and requires calibration to establish accurate conversions, highlighting the limitations of relying solely on OD for quantitative comparisons across experiments and species.

How much bacillus sp drops during one month storage after liofilisation?4 answersThe viability of Bacillus sp. during one month storage after liofilisation varies depending on the specific strain and storage conditions. In one study, Bacillus sp. AA2 and Bacillus sp. MER had a viability of 1.9×10^6 cfu mL^-1 and 1.2×10^6 cfu mL^-1, respectively, after storage for an unspecified period of time. Another study found that the number of viable bacteria cells in a vermiculite-based formulation declined to 71% after 9 months of storage at 10 and 20°C. However, the specific decrease in viability during one month storage after liofilisation was not mentioned in the abstracts provided. Further research is needed to determine the exact decrease in viability of Bacillus sp. during one month storage after liofilisation.

How to calculate cell density from microscope image?5 answersCell density can be calculated from microscope images using various methods described in the literature. One approach involves developing a tool to assess total cell numbers in a microscope's field of vision, which provides the denominator for calculating the percentage of positive cells for a given antigen. Another method uses an algorithm to estimate cell density from still intensity images captured by an in-situ microscope. This algorithm segments image regions containing cells and estimates the cell density inside each segmented region. A different technique involves counting the number of cells sectioned by a line of known length on a micrograph to determine average cell size and cell density. Additionally, an intracellular density imaging technique based on ratiometric stimulated Raman scattering microscopy has been introduced, which allows for real-time measurement of intracellular density and differentiation of cell types. Another method involves using image identification and manual correction to automatically identify and count cone cells in visual images, enabling cone-cell density calculation.

What are the mechanisms underlying the decrease in optical density during spore germination?4 answersThe decrease in optical density during spore germination is influenced by several mechanisms. One of the mechanisms is the activation of germination receptors, which triggers the germination process. Another mechanism is the release of ions, such as calcium and chloride, which act as essential germinants for spores. The presence of nutrients, such as those found in apple juice, can also promote spore germination. Additionally, the temperature and the degree of heat activation can affect the kinetics of germination, including the rate of decrease in optical density. These mechanisms collectively contribute to the observed decrease in optical density during spore germination.

How can optical density be used to estimate the cell concentration of Bacillus sibitulis?5 answersOptical density can be used to estimate the cell concentration of Bacillus sibitulis. Different wavelengths of light can be used to measure optical density, and the choice of wavelength depends on the organism and the culture conditions. For pigmented organisms like Bacillus sibitulis, the choice of wavelength should consider both robustness and sensitivity of the measurement, taking into account light scattering and absorption by pigments. By comparing the organism's spectrum to the true absorption spectra of dyes, suitable wavelengths can be identified. Additionally, the relative scattering contribution can be reduced by measuring at higher optical density or by adding bovine serum albumin. The accuracy of optical density-based measurements is dependent on the spectrophotometer model, wavelength, and culture conditions. Therefore, it is important to consider these factors when using optical density to estimate the cell concentration of Bacillus sibitulis.

What is the optical density of dermatophytes for mc farland?4 answersThe optical density of dermatophytes for mc farland was not mentioned in any of the provided abstracts.

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