What are the advantages and disadvantages of using SNPs for mapping X chromosome inactivation?
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Using single-nucleotide polymorphisms (SNPs) for mapping X chromosome inactivation (XCI) has several advantages and disadvantages. SNPs offer higher resolution mapping compared to multiallelic markers like STRPs, allowing for more precise identification of mutations and chromosomal breakpoints . SNPs also enable the assessment of allele-specific gene expression in normal human cells, providing insights into X-linked gene activity . However, the use of SNPs for XCI mapping requires additional analytical and computational challenges due to the densely spaced diallelic markers . Additionally, the analysis of XCI patterns using SNPs may not reflect what happens in a normal human cell, as it has been primarily studied in somatic cell hybrids . Therefore, while SNPs offer advantages in terms of resolution and gene expression analysis, careful consideration of the limitations and challenges associated with their use is necessary for accurate mapping of XCI.
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