What are the effects of heat on plasmid curing in Proteus?
Best insight from top research papers
Heat has been shown to have an effect on plasmid curing in Proteus. In one study, it was found that incubation at elevated temperatures resulted in the curing of a large plasmid in a fast-growing Rhizobium sp. from Lablab purpureus . Another study focused on Proteus isolates recovered from clinical specimens and found that plasmid curing by acridine orange and elevated temperature resulted in the loss of invasiveness and adhesion capabilities, indicating that these virulence factors are plasmid-encoded .
Answers from top 4 papers
More filters
Papers (4) | Insight |
---|---|
86 Citations | The effects of heat on plasmid curing in Proteus are not mentioned in the provided paper. |
14 Citations | The provided paper is about the effects of curing agents on phenotypic stability in Pseudomonas putida, not Proteus. Therefore, there is no information in the paper about the effects of heat on plasmid curing in Proteus. |
27 Citations | The provided paper is about plasmid curing in an acidophilic bacterium of the genus Acidocella, not Proteus. Therefore, there is no information in the paper about the effects of heat on plasmid curing in Proteus. |
01 Dec 2009 | The effects of heat on plasmid curing in Proteus are not mentioned in the provided paper. The paper discusses the distribution of virulence factors in Proteus isolates and their correlation with invasion and cytotoxicity. |
Related Questions
What are the factors affecting the heat resistance of bioplastics?5 answersThe heat resistance of bioplastics can be influenced by various factors. One crucial factor is the addition of metal compounds like aluminum oxide, which acts as an additive to enhance heat resistance in bioplastics. Mold conditions, such as mold temperature and cooling time, also play a significant role in improving the heat resistance of injection-molded bioplastic products. Furthermore, controlling injection molding parameters like mold temperature and cooling time can enhance the crystallinity and crystal form of bioplastics, thereby improving their heat resistance. Additionally, the composition of bioplastic blends, such as polyhydroxybutyrate, polylactic acid, and poly(ε-caprolactone), can impact the mechanical properties and heat resistance of the resulting bioplastic films. These factors collectively contribute to enhancing the heat resistance of bioplastics for various applications.
What are the specific molecular mechanisms by which anti-plasmid therapy works to treat bacterial infections?5 answersAnti-plasmid therapy for treating bacterial infections operates through various molecular mechanisms. Firstly, compounds like phenothiazines destabilize plasmid DNA replication, inhibit partitioning during cell division, and block conjugation, effectively eliminating plasmids from antibiotic-resistant bacteria. Additionally, heterocyclic compounds bind to plasmid DNA, reversing antibiotic resistance and virulence in bacterial strains. Promethazine and 9-aminoacridine have been shown to eliminate plasmids from enteric bacteria, reducing doxycycline resistance. Plasmids, which carry antibiotic-resistant genes, are crucial targets for therapy, as they contribute significantly to antibiotic resistance in bacterial infections. These findings underscore the importance of understanding and targeting plasmids to combat antimicrobial resistance effectively.
What is the influence of a heat shock during sporulation in the resistance to wet heat of bacterial spores?5 answersA heat shock during sporulation significantly influences the resistance of bacterial spores to wet heat. The presence of a protein called 2Duf enhances spore resistance to wet heat and various chemicals that act on or cross the spores' inner membrane (IM). Spores made at higher temperatures with 2Duf exhibit increased resistance to wet heat and chemicals. Additionally, the high heat resistance of dormant bacterial spores is linked to the extent of protoplast dehydration and the concentration of dipicolinic acid (DPA) in the spore core. The dynamics of water in hydrated DPA and Ca-DPA within the spore core play a crucial role in the spores' resistance to moist heat. These findings collectively highlight the intricate relationship between heat shock during sporulation and the subsequent resistance of bacterial spores to wet heat.
How does temperature impact the phage-host interaction?4 answersTemperature has a significant impact on the phage-host interaction. The bacteriophage Qβ adapts to variations in host density by selecting different mutations in response to changes in environmental temperature. The C22 phage infects the host cell more effectively at low temperatures compared to high temperatures, and this is attributed to changes in phage stiffness and attachment to the cell surface. Prophages in the bacterium Serratia marcescens undergo temperature-dependent release, which can lead to modified bacterial virulence in an insect host. Experimental evolution under different temperatures also results in genetic and epigenetic changes in the bacterial host, influenced by the temperature-dependent induction of a temperate phage. These findings highlight the importance of considering temperature as a key factor in understanding the dynamics of phage-host interactions.
Why heat shock is required for the introduction of the hybrid vector into the host cell?5 answersHeat shock is required for the introduction of the hybrid vector into the host cell because it plays a role in increasing or decreasing virus transmission. Heat shock proteins (HSPs) are involved in this process, as they help in protein folding, assembly, and degradation. The elevated synthesis of HSPs during heat shock is essential to prevent non-native proteins from misfolding and aggregation. HSPs also protect the vector's midgut from translocating the virus to the hemolymph, thereby reducing virus transmission. In addition, HSPs are involved in differentiation, protection from the host cell's killing mechanisms, and virulence in parasites. Therefore, heat shock and the induction of HSPs are crucial for the successful introduction of the hybrid vector into the host cell, as they facilitate protein folding, protect against host defenses, and enhance virus transmission.
How many plasmids can a yeast cell tolerate?5 answersYeast cells can tolerate multiple plasmids. The study of yeast DNA plasmids has shown that they can exist in the nucleus and be organized into chromatin structure in vivo. Yeast transformation with plasmids has been used to isolate and characterize various genes, ars, centromeres, and telomeres. Different yeast plasmids have been screened among phylogenetically distant yeasts, indicating the potential for diverse plasmid systems. The use of dicentric plasmids in yeast cells has also been observed, with rearrangements involving deletion of centromere sequences. The presence of multiple plasmids can lead to activator titration and background expression. The availability of low-copy (ARS/CEN) and multi-copy (2-μm-based) plasmids has provided options for stable maintenance of plasmids in yeast cells. The number of plasmids a yeast cell can tolerate depends on factors such as plasmid stability and gene expression.