What are the mechanisms by which saturated fatty acids contribute to the structural integrity of nanoparticles?
Best insight from top research papers
Saturated fatty acids contribute to the structural integrity of nanoparticles through various mechanisms. They can affect protein conformation, fatty acid binding, and protein aggregation. Additionally, the high affinity and stable binding characteristics of fatty acids to albumin specific sites play a crucial role in preparing nanoparticles with increased drug content and stability. The presence of counterions can influence the adsorption and self-assembly of fatty acids on nanoparticle surfaces, leading to changes in morphology and interfacial activity, ultimately impacting stability. Moreover, in the presence of vesicles or cell membranes, lipid adsorption on nanoparticles can result in the formation of supported lipid bilayers, which resist cellular uptake and can lead to the encapsulation/release of drugs/DNA. Covalent conjugation between fatty acid-capped nanoparticles and proteins further enhances structural integrity, as seen in the development of nanobioconjugates.
Answers from top 4 papers
More filters
| Papers (4) | Insight |
|---|---|
| Saturated fatty acids, like stearic acid, covalently bind to nanoparticles via fatty acid binding domains on proteins, enhancing stability and structural integrity of the nanobioconjugate. | |
12 Citations | Saturated fatty acids contribute to nanoparticle integrity by forming lipid sheaths around supported lipid bilayers, enhancing structural stability and resisting cellular uptake. |
24 Citations | The addition of ethanolamine as a counterion alters the morphology of decanoic acid on aminopropyl-modified silica nanoparticles, transitioning from discrete patches to a continuous bilayer, impacting foam stability. |
| Saturated fatty acids influence protein aggregation and conformation on nanoparticles by affecting fatty acid binding, protein stability, and uniformity of binding sites, enhancing structural integrity. |
Related Questions
How do nanoparticles influence bacterial growth mechanisms?5 answersNanoparticles have a significant impact on bacterial growth mechanisms. They can act as bactericides, disrupting cell membranes and inhibiting microbial growth. Furthermore, metal oxide nanoparticles like Cu2O, Fe3O4, and Ag2O alter soil bacterial communities by reducing diversity and shifting abundance towards specific bacterial groups, potentially due to their antimicrobial properties. Nanoparticles, such as n-ZnO and n-TiO2, affect bacterial physiology by influencing metabolic pathways related to stress responses, oxidative stress, and stringent responses, ultimately impacting the ability of bacteria like Bacillus subtilis to resist stress. Utilizing nanoparticles as quorum quenchers against human pathogenic bacteria presents a promising strategy to combat multidrug resistance and virulence, offering an efficient alternative to traditional antibiotics with minimal resistance development.
What is the need of biosurfactant coating on nanoparticles?5 answersThe coating of nanoparticles with biosurfactants serves multiple crucial purposes. Biosurfactants, derived from microorganisms and plants, act as stabilizers and capping agents for nanoparticles, preventing aggregation and maintaining formulation stability. They reduce the formation of aggregates during nanoparticle synthesis, ensuring a uniform morphology and enhancing biocompatibility. Biosurfactants also play a significant role in reducing metal precursors and stabilizing nanoparticles, contributing to their eco-friendly and non-toxic nature. Moreover, biosurfactants can enhance the mobility of nanoparticles through porous media, promoting their transport characteristics and interactions with the environment. Overall, the use of biosurfactant coatings on nanoparticles is essential for improving stability, biocompatibility, and environmental interactions, making them valuable in various fields such as nanotechnology, bioremediation, and pharmaceuticals.
What are the common stabilizers for nanoparticles?3 answersPolymeric stabilizers are commonly used for nanoparticles. In the study by Derikov et al., diblock copolymers were found to be the most effective stabilizers for composites based on a diblock copolymer of styrene and 2-vinyl pyridine and spherical Au nanoparticles. Balestri et al. explored the use of a thermo-responsive copolymer poly(N,N-dimethylacrylamide)-block-poly(N-isopropylacrylamide) (PDMA-b-PNIPAM) as a stabilizer for lipid-based nanoparticles for drug delivery. Prud'homme et al. described the formation of core-shell-brush nanoparticles using an A-B-C triblock copolymer, where the A block acts as a stabilizing brush layer. Prajapati and Serajuddin used sugar esters as nanoparticle stabilizers in solid dosage forms. Capek discussed the use of various methods and reductants, such as surfactants and polymer coatings, for stabilizing noble metal nanoparticles.
Which method is used for the formulation of conventional lipid nanoparticles?4 answersThe method used for the formulation of conventional lipid nanoparticles is the mixing of an aqueous solution with a solid lipid matrix and a biocompatible non-ionic surfactant, followed by heating and cooling processes. This method allows for the production of solid lipid nanoparticles with a size of less than 500 nm. These lipid nanoparticles are composed of biodegradable lipids such as triglycerides, fatty acids, fatty alcohols, waxes, and butter, which turn solid at room temperature. The production of lipid nanoparticles can also be achieved through freeze-drying or spray-drying techniques, which result in a dry powder formulation suitable for pharmaceutical applications. These lipid nanoparticles offer advantages such as high stability, biodegradability, and the ability to incorporate a wide variety of drugs for effective targeting.
What are the different mechanisms by which nanoparticles can enter cells?5 answersNanoparticles can enter cells through various mechanisms. One mechanism is active endocytosis, where cells use different pathways to generate membrane curvature for nanoparticle uptake. Another mechanism is passive diffusion, where nanoparticles can passively diffuse across the lipid bilayer of the cell membrane. Additionally, nanoparticles can enter cells through clathrin-independent mechanisms, which do not involve the clathrin-mediated pathway typically observed for receptor-mediated endocytosis. Nanoparticles can also be internalized via receptor-mediated endocytosis, where they interact with specific receptors on the cell surface, such as the LDL receptor. Furthermore, nanoparticles can enter cells through active transport mechanisms, including microtubule-involved endocytosis. The size, shape, surface functional groups, and elasticity of nanomaterials can also affect their endocytosis into cells. Overall, understanding the different mechanisms of nanoparticle uptake into cells is crucial for designing effective drug delivery systems and improving biomedical applications.
How to characterize lipid nanoparticles?5 answersLipid nanoparticles (LNPs) can be characterized using various analytical tools. One common approach is the use of atomic force microscopy (AFM) to visualize the morphology of LNPs. AFM provides detailed information about the nanostructure of LNPs and can be used to visualize drug-loaded LNPs. Another method is dynamic light scattering (DLS), which is used to determine the average diameter and size distribution of LNPs. DLS analysis can help assess the stability of LNPs and provide information about their size. Flow cytometry is another technique that can be used to quantify and assess LNPs. It allows for the simultaneous measurement of size and structure of individual vesicles, providing high throughput analysis. Additionally, electron paramagnetic spectroscopy combined with molecular dynamics simulations can provide insights into the structure and dynamics of lipids in lipid nanodiscs. These techniques, along with others, contribute to our understanding and characterization of LNPs.