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What is Spectrophotometry? 


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Spectrophotometry is a technique used to measure the interaction between electromagnetic radiation and matter. It involves the absorption of different frequency components of radiation by the matter . Spectrophotometry is commonly used in various fields such as semiconductor manufacturing and research on optic-matter interaction . It is used for identification and quantification of raw materials and pharmaceutical products . Spectrophotometers can measure the thickness, refractive index, and other properties of different layers on wafers . They can also measure the absorbance and transmittance of liquid solutions within the visible range of the electromagnetic spectrum . Spectrophotometers can be used to detect process drifts and correct concentration changes caused by temperature differences . Additionally, they can be used to measure the fluorescence characteristics of samples, improving quantification accuracy .

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Spectrophotometry is the analysis of the interaction between light and matter, specifically the measurement of absorbance and transmittance of liquid solutions within the visible range of the electromagnetic spectrum.
Book ChapterDOI
01 Jan 2020
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Spectrophotometry is a method used to measure the interaction of electromagnetic radiations with matter, specifically the absorption of light by different degrees of freedom of matter and/or substance.
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