What is the current trend in the use of Caco-2 cell assay in human health risk assessment ?
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The current trend in the use of Caco-2 cell assays in human health risk assessment involves advancements in various areas. Researchers have successfully developed miniaturized 96-well formats for Caco-2 assays, enhancing efficiency and capacity . Additionally, Caco-2 cell bioassays are being utilized for assessing iron bioavailability in foods, providing a cost-effective and high-throughput approach . Moreover, Caco-2 cell assays are gaining attention for evaluating antioxidant activity in foods, bridging the gap between in vitro and in vivo studies . Efforts to enhance the predictive accuracy of drug absorption and metabolism are ongoing, with the introduction of artificial chromosome vectors to increase enzyme expression in Caco-2 cells . Furthermore, the development of mucin-protected Caco-2 assays in complex biorelevant media is enabling a better understanding of drug permeation and addressing biopharmaceutical questions, such as food effects on oral drug absorption .
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| Papers (5) | Insight |
|---|---|
| The current trend involves enhancing Caco-2 assays with mucin and biorelevant media to study drug permeation, addressing challenges like poor solubility and food effects in human health risk assessment. | |
| The current trend involves enhancing Caco-2 cells with multiple CYPs via a mammalian artificial chromosome to improve drug metabolism prediction, aiding in human health risk assessment. | |
18 Citations | The current trend in human health risk assessment involves utilizing Caco-2 cell assay for evaluating antioxidant activity in foods, aiding in determining biological effects and bioavailability. |
2 Citations | The current trend involves optimizing Caco-2 cells for a miniaturized 96-well permeability assay, enhancing efficiency and capacity for evaluating compound permeability and transporter interactions in human health risk assessment. |
3 Citations | The Caco-2 cell bioassay is utilized for assessing food iron bioavailability, providing a cost-effective and high-throughput method for evaluating nutritional quality, rather than human health risk assessment. |
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