How can hydrogen peroxide induce oxidative stress in hela cells?4 answersHydrogen peroxide (H2O2) induces oxidative stress in HeLa cells through various mechanisms. H2O2 inhibits rDNA transcription, reduces RNA content, and causes nucleolar protein relocalization. It increases ROS levels, activates caspase-3, and induces apoptosis or necrosis, leading to mitochondrial membrane potential loss. Additionally, H2O2 can modulate aquaporin (AQP) permeability, facilitating H2O2 diffusion and increasing oxidative stress. Moreover, H2O2 can enhance hydrogen peroxide production by lactate dehydrogenase (LDH), contributing to cellular oxidative stress. Conversely, U. rigida ethanolic precipitate protects against H2O2-induced apoptosis by upregulating anti-apoptotic Bcl-xL and preventing MMP loss. Overall, H2O2 induces oxidative stress in HeLa cells by disrupting cellular processes, increasing ROS levels, and promoting apoptotic pathways.
How does hydrogen peroxide affect seed germination?4 answersHydrogen peroxide (H2O2) has been found to have beneficial effects on seed germination. It has been shown to alleviate reduced germination percentage and seedling growth under drought stress in sorghum. H2O2 treatment increases energy production and defense activity, leading to an increase in germination rate and seedling growth. In barley seeds, H2O2 treatment, along with plasma treatment, accelerates the germination rate by affecting the abscisic acid factor. In sugarbeet seeds, H2O2 treatment improves germination by overcoming physical and physiological dormancy. Reactive oxygen species (ROS), including H2O2, play a critical role in regulating seed germination by modulating seed maturation, dormancy, and viability. H2O2 and Ca2+ signaling crosstalk offsets abscisic acid (ABA) to induce seed germination by maintaining a balance between ABA and gibberellic acid (GA3).
What percentage of hydrogen peroxide is found in paper industry wastewater?5 answersThe percentage of hydrogen peroxide found in paper industry wastewater is not mentioned in the abstracts provided.
How does hydrogen peroxide affect enzyme activity?4 answersHydrogen peroxide (H2O2) affects enzyme activity by inhibiting the capping enzyme (HCE) and oxidizing the active site cysteine residue of the HCE triphosphatase domain. H2O2 also modulates the activity of thimet oligopeptidase (TOP), a cytosolic zinc protease, by increasing its activity in certain conditions. Additionally, H2O2 can be used to engineer enzymes for enhanced production of H2O2, as shown in the study with cytochrome P450 BM3 variants. The presence of H2O2 causes oxidative stress in algal cells, leading to decreased cell growth, photosynthetic activity, and metabolic activity ^[Context_1,. The increased activities of superoxide dismutase (SOD) and catalase (CAT) indicate the cells' response to H2O2-induced oxidative stress ^[Context_1,. Overall, H2O2 has a significant impact on enzyme activity, both inhibiting and modulating the activity of various enzymes.
What are the effects of hydrogen peroxide on plants?4 answersHydrogen peroxide (H2O2) has various effects on plants. It acts as a metabolic signal, improving ion homeostasis and increasing salt tolerance in plants. H2O2 foliar spraying can stimulate physiological processes in leaves, but higher doses can lead to stress and decrease pigment levels, photosynthetic attributes, and antioxidant capacity. Exogenous application of H2O2 plays a pivotal role in regulating plant metabolism under stress conditions, improving growth, photosynthetic capacity, and antioxidant protection. H2O2 signaling balances plant metabolism through cross-talk with other signals and hormones, and it regulates stress-responsive transcription factors for stress tolerance. At low nanomolar concentrations, H2O2 acts as a signaling molecule in plants, resembling phytohormones and playing an indispensable role in plant growth, development, and crosstalk with other signaling pathways.
How does soaking seeds in a hydrogen peroxide solution affect germination?5 answersSoaking seeds in a hydrogen peroxide solution has been shown to have a positive effect on germination. It increases germination percentage, germination rate, vigor index, plumule and radicle length, soluble carbohydrates and protein contents, and antioxidant enzyme activities such as catalase, ascorbate peroxidase, and superoxide dismutase. Additionally, soaking seeds in hydrogen peroxide enhances stomata density, leaf length, and histological components, resulting in higher fresh and dry weights, seed number per spike, and grain yield. Hydrogen peroxide also helps overcome the adverse effects of hypoxia on germination, promoting hypocotyl elongation and stem growth. Furthermore, a method involving soaking carrot seeds in a hydrogen peroxide solution has been developed to accelerate germination, resulting in quick and robust seedling emergence. Overall, soaking seeds in a hydrogen peroxide solution improves germination and seedling performance by enhancing physiological and biochemical processes.