What is the role of plant tissue culture in agricultural and industrial biotechnology?
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Plant tissue culture plays a crucial role in agricultural and industrial biotechnology. It is used for mass production of crops with improved traits, such as food, feed, fiber, and fuel . Plant tissue culture techniques are employed for various purposes, including micropropagation, functional gene studies, transgenic plant production, and crop improvement . It allows the production of exact copies of plants with desirable traits and the regeneration of whole plants from genetically modified cells . Additionally, plant tissue culture is used for the production of plants in sterile containers, reducing the risk of transmitting diseases and pests . It also enables the production of plants from seeds with low germination rates and the conservation of germplasm of vegetatively propagated plant crops . Furthermore, plant tissue culture is essential for the production of pathogen-free plants, secondary metabolite production, and understanding various aspects of plant science .
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Open access•Journal Article | The role of plant tissue culture in agricultural and industrial biotechnology includes plant micropropagation, generation of transgenic plants, crop improvement, virus elimination, and preservation of germplasm. |
| Plant tissue culture is used in agricultural and industrial biotechnology to increase the number of elite plant lines, generate new varieties, and produce valuable metabolites through culture mutation or transformation. | |
| The paper discusses the role of plant tissue culture in agricultural biotechnology, including its contribution to the production of crops with improved food, feed, fiber, and fuel. It also mentions that tissue culture is a powerful technique for mass production in many crops and has become an important tool in the nursery and farming industry. However, it does not specifically mention the role of tissue culture in industrial biotechnology. | |
| Plant tissue culture techniques have revolutionized the agricultural sector by producing mature plants quickly, cloning plants with desirable traits, and developing genetically modified plants. |
Related Questions
How tissue culture is involved in the production of transgenic cows?5 answersTissue culture plays a crucial role in the production of transgenic cows by providing a platform for genetic modification and subsequent cloning. In the context of transgenic cattle production, tissue culture techniques are utilized for various purposes such as introducing transgenes into bovine cells, ensuring stable integration of the foreign DNA into the genome, and promoting the development of cloned embryos. These techniques involve processes like lipofection of primary fibroblasts with gene expression cassettes, somatic cell nuclear transfer (SCNT) for cloning transgenic fibroblasts, and optimizing culture media to enhance the efficiency of somatic cell nuclear transfer protocols. Through tissue culture methods, researchers can generate transgenic cows expressing desired traits or proteins, offering opportunities for advancements in agriculture, biomedicine, and food production.
What are the recent studies about plant tissue culture in philippines?4 answersPlant tissue culture studies in the Philippines have focused on various plant species and their growth parameters. One study investigated the growth of Dillenia philippinensis shoot tip cultures on different types and concentrations of cytokinins, finding that they significantly affected growth parameters. Another study identified and characterized fungal contaminants in abaca tissue cultures, highlighting the challenges of contamination in plant tissue culture. A study on mango tissue culture developed a protocol for regeneration and successful transfer of plantlets to soil, enabling rapid propagation of superior strains. Permaculture farms in the Philippines were found to cultivate a diverse range of plant species, primarily for household consumption. Mass propagation of Dillenia philippinensis was achieved through tissue culture using mature seeds as explants, with successful acclimatization and survival of plantlets.
Why are plant cell suspension cultures used in biotechnology and pharmaceutical research?4 answersPlant cell suspension cultures are used in biotechnology and pharmaceutical research for several reasons. Firstly, they provide an easy and scalable platform for the production of high-value compounds such as therapeutic proteins and bioactive compounds. These compounds are often challenging to synthesize chemically and can be produced in meager quantities in natural plants. Plant cell cultures offer a way to retain the metabolic cues of natural plants and create high-yielding cell factories for the synthesis and accumulation of targeted metabolites. Additionally, plant cell cultures allow for the manipulation and modification of synthesis pathways, leading to the improvement of metabolite biosynthesis. Furthermore, plant cell cultures offer advantages such as low cost, environmental friendliness, and the potential for precise genetic engineering using tools like CRISPR/Cas9. Overall, plant cell suspension cultures are valuable tools in biotechnology and pharmaceutical research for the production of high-value compounds in a safe and efficient manner.
What is the significance of cell culture?5 answersCell culture is a crucial tool in understanding various aspects of cells and tissues, such as cell biology, disease mechanisms, drug action, and cancer research. It allows for the growth and reproduction of cells in a controlled environment, mimicking in vivo conditions. There are two major types of cell cultures: two-dimensional (2D) and three-dimensional (3D) cultures. 2D culture, which has been used since the 1900s, is simple and cost-effective, forming a monolayer. On the other hand, 3D culture is an advanced version that includes models and spheroid structures with multiple layers of cells, and it is currently being extensively studied. Cell culture has various applications, including studying cell motility, disease treatment, drug discovery, and development. Additionally, it plays a significant role in preclinical trials of drugs and the production of therapeutic proteins.
How does plant tissue culture work?5 answersPlant tissue culture is a technique used to produce plants in a controlled environment, independent of climatic and geographical conditions. It involves the growth of plant cells, tissues, or organs in a nutrient-rich medium under sterile conditions. This technique is used to produce high-quality, disease-free, and true-to-type plants at a fast rate. The process of plant tissue culture includes the reprogramming of explants, which undergo morphological, physiological, biochemical, genetic, and epigenetic changes. These changes can be induced by oxidative stress, nutrient components, and environmental factors. Organic growth additives can be incorporated into the culture media to stimulate tissue growth and increase the number of shoots, leaves, and roots. The genetic stability of in vitro regenerated plants can be assessed using DNA-based molecular markers. Plant tissue culture is an important tool for the continuous production of active compounds, including secondary metabolites and engineered molecules, which are used in pharmaceutical industries, cosmetics, dietary supplements, and other industrial sectors.
What are the challenges associated with tissue culture of potato?5 answersTissue culture of potato faces several challenges. One challenge is the low rate of multiplication and high risk of diseases associated with using seed potato tubers for propagation. Another challenge is the accumulation and dissemination of pathogens when using tubers for asexual propagation, which can negatively impact yield and threaten the maintenance of genotypes. Additionally, ethylene accumulation in the culture vessel can affect potato tissue culture, and while silver nanoparticles have been shown to inhibit ethylene action, their application in potato tissue culture is not well-studied. Furthermore, there is a need for efficient and effective tissue culture methods, such as the use of a multi-station treatment structure, to improve the culture treatment efficiency and save manpower. Lastly, the addition of pesticides to the culture medium can improve the finished product rate of tissue culture seedlings and reduce production costs.