What primer pairs are used for ngs of plant endophytes?
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The primer pairs used for next-generation sequencing (NGS) of plant endophytes include 799F/1107R, 322F/796R, and 322F-Dr/796Rs, designed to specifically amplify bacterial 16S from plant total DNAs . Additionally, primer pair 322F-1 and 796R, along with DNA polymerase lacking 3' to 5' exonuclease proofreading activity, are utilized to avoid contamination from host plant chloroplast 16S rDNA and mitochondria 18S rDNA, resulting in a pure bacteria colony 16S amplicon library . Furthermore, novel primers fM1 and rC5, along with 515f-GC/rC5, are employed for nested PCR denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the diversity of endophytic bacteria in Dendrobium officinale, revealing Proteobacteria as the dominant group, particularly Burkholderia, which may play essential roles in the plant .
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58 Citations | The PCR assay in the study used specific primers flanking a microsatellite-containing locus (MS primers) for detection and quantification of Epichloe endophytes in grass tissue. |
| Novel 16S rDNA-targeted primers fM1 and rC5, along with 515f-GC/rC5, are used for nested PCR-DGGE to analyze endophytic diversity in Dendrobium officinale. | |
6 Citations | The primer pairs used for NGS of plant endophytes are 799F/1107R, 322F/796R, and 322F-Dr/796Rs, designed to specifically amplify bacterial 16S from plant total DNAs, enabling accurate sequencing. |
Patent 04 Jun 2014 4 Citations | The primer pairs used for amplification of endophytic fungi ITS gene in plant tissue are NSA3 and NLC2 for the first round, followed by a nested PCR second round amplification primer pair. |
| The primer pair 322F-1 and 796R is used for NGS of plant endophytes, targeting the V3 to V4 region of bacteria 16SrDNA to avoid contamination from plant mitochondria and chloroplast DNA. |
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