What role does geography play in the development and implementation of RT-PCR detection methods for dengue virus?
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Geography plays a significant role in the development and implementation of RT-PCR detection methods for dengue virus. The wide and rapid distribution of the virus is influenced by factors such as increased urbanization, migration, global commerce, weather variation, inadequate mosquito control, and shifting climatic circumstances . These factors vary across different geographical regions, leading to the need for adaptable and accessible detection protocols. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) method has emerged as a suitable viral detection procedure due to its simplicity, sensitivity, and cost-effectiveness . Additionally, the use of real-time multiplex RT-PCR has been found to be more sensitive in detecting dengue virus coinfections compared to conventional multiplex RT-PCR . Therefore, the development and implementation of RT-PCR detection methods for dengue virus need to consider the specific geographical characteristics and challenges of each region to ensure accurate and timely detection of the virus .
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