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What should be thecurrent, power and voltage for PAGE gel electrophoresis?

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For Polyacrylamide Gel Electrophoresis (PAGE), the current, power, and voltage requirements vary based on the setup. In the context of electrophoresis power supplies, a controllable current of 0-90 mA at 120 V AC power supply is achievable at a low cost. Additionally, a study on an adapted electrophoresis power supply achieved 300V DC at 200mA for effective electrophoretic functions. To enhance efficiency, using Sodium Tetraborate in gel electrophoresis buffers allows for higher voltages, like 35 volts/cm, reducing run times significantly. In the context of RVSG apparatus for protein separation, a setup with 120 V for 6 hours at 25°C was used successfully for isoenzyme studies. Therefore, for PAGE gel electrophoresis, a current range of 0-200mA, power consumption based on the setup, and voltages up to 35 volts/cm can be considered depending on the specific requirements.

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Patent Electrophoresis electrical power unit Gao Wenxiu, Zhao Baitong, Li Shuai - Show less +2 more 08 Feb 2017 1 Citations	The electrophoresis electrical power unit can provide a wide range of voltage and current outputs, allowing for precise control of parameters like current, power, and voltage required for PAGE gel electrophoresis.
Open access•Journal Article An inexpensive method for denatured and native multigel in a regular vertical slab gel system Sushanta Kumar Saha, A. Kalib, Lakshmanan Uma, Dharmar Prabaharan, G. Subramanian - Show less +4 more 01 Jan 2003-Current Science 2 Citations	For PAGE gel electrophoresis, a current of 120 V for 6 hours at 25°C was used in the study, with proteins stacked at 60 V for 3 hours. Power can be calculated using Ohm's Law.
Posted Content•DOI Preparing Borax RapidBuffer for electrophoresis v1 01 Mar 2022	For PAGE gel electrophoresis using Borax RapidBuffer, run at 35 volts/cm, 200V, and adjust current based on gel size for efficient separation of DNA fragments.
Journal Article•DOI Economical electrophoresis with a controlled-current power supply David R. Hartman, William H. Courtney - Show less +1 more 01 Aug 1990-Journal of Chemical Education 3 Citations	For PAGE gel electrophoresis, the electrophoresis power supply provides a controllable current of 0-90 mA, with a voltage of approximately 180-375 V, and a power determined by the load resistance.
Open access Construction and Testing of Electrophoresis Power Supply Ezeonu S. O, Okonkwo G. N - Show less +1 more 01 Jan 2011	The electrophoresis power supply in the study delivered 300V at 200mA, suitable for PAGE gel electrophoresis, ensuring efficient separation of biomolecules.

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