Where to find detailed protocol of cfDNA extraction from blood with magnetic beads?
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A detailed protocol for cell-free DNA (cfDNA) extraction from blood using magnetic beads can be found in a study by Seong et al. The method involves utilizing homobifunctional crosslinkers, such as dimethyl suberimidate dihydrochloride (DMS), in combination with amine-conjugated magnetic beads to efficiently isolate cfDNA from plasma within 10 minutes. This approach demonstrates a 56% higher extraction efficiency compared to commercial kits like the QIAamp kit, highlighting its effectiveness and rapid processing time . Additionally, Oberacker et al. provide insights into the general use of magnetic beads for nucleic acid purification, emphasizing their cost-effectiveness, versatility, and scalability for various DNA and RNA extraction applications, including bisulfite conversion and size selection of fragments .
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| The detailed protocol for cfDNA extraction from blood using magnetic beads can be found on the BOMB platform website (https://bomb.bio). | |
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| The provided paper does not provide a detailed protocol for cfDNA extraction from blood with magnetic beads. | |
| The detailed protocol for cfDNA extraction from blood using magnetic beads can be found in the research paper "Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers." |
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