Are there any published protocols for extracting bacterial extracellular vesicles from bacterial cultures?
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There are published protocols for extracting bacterial extracellular vesicles (BEVs) from bacterial cultures. One protocol involves using a broad-spectrum antimicrobial epsilon-poly-L-lysine (ϵ-PL) to precipitate BEVs at a relatively low centrifugal speed, followed by recovery and buffer exchange through pH and ionic strength adjustment and ultrafiltration . Another protocol utilizes a label-free and biocompatible on-chip magnetic separation system to efficiently extract small vesicles (sEVs) from cell culture supernatant. This system generates a magnetic field gradient inside the separation microchannel, allowing for the separation of sEVs from other bioparticles. The recovered sEVs have high purity and recovery rates, surpassing the performance of the ultracentrifugation method . These protocols provide efficient and cost-effective methods for isolating and enriching BEVs and sEVs from bacterial cultures, enabling further studies on their compositions and biological functions.
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| Papers (5) | Insight |
|---|---|
| The provided paper does not mention any published protocols for extracting bacterial extracellular vesicles from bacterial cultures. | |
| The paper discusses three different methods for isolating extracellular vesicles (EVs) from bacterial cultures: direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC). | |
9 Citations | The provided paper does not mention any protocols for extracting bacterial extracellular vesicles from bacterial cultures. |
| Yes, the paper provides a published protocol for extracting bacterial extracellular vesicles from bacterial cultures using epsilon-poly-L-lysine (ϵ-PL). | |
| The provided paper does not mention any published protocols for extracting bacterial extracellular vesicles from bacterial cultures. |
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