scispace - formally typeset
Search or ask a question

Whereas DNA isolation may involve the removal of RNA 


Best insight from top research papers

DNA isolation may involve the removal of RNA. The present invention describes methods for removing DNA from an RNA transcript during the mRNA production process . RNA, preferably messenger RNA, can be purified by selective precipitation, which can remove both RNA and DNA, leaving behind a mixture advantageous for further purification . Most procedures for isolating RNA from eukaryotic cells involve additional steps to remove DNA . Furthermore, sequencing-based analyses have shown the presence of small RNA contaminants in microRNA extraction kits, highlighting the need for their depletion to avoid misinterpretation in sRNA studies .

Answers from top 5 papers

More filters
Papers (5)Insight
The paper does not specifically mention the removal of RNA during DNA isolation. The paper is about the detection and removal of small RNA contaminants in microRNA extraction kits.
The provided paper does not mention the removal of RNA during DNA isolation. The paper is about the preparation of RNA from tissues and cells.
The provided paper does not specifically mention the removal of RNA during DNA isolation. The paper focuses on the isolation of RNA from tissues and cells.
The paper discusses a method for selectively precipitating DNA away from RNA, suggesting that DNA isolation may involve the removal of RNA.
The provided paper does not discuss the removal of RNA during DNA isolation. The paper focuses on methods of removing DNA from an RNA transcript during the mRNA production process.

Related Questions

Why is RNA extracted from transfected cells?4 answersRNA is extracted from transfected cells to study and analyze the genetic material of the cells. This extraction allows researchers to investigate the replication and translation processes of positive stranded RNA viruses, such as Classical swine fever virus (CSFV). Additionally, RNA extraction from transfected cells is used in the development of therapeutic vaccines for human immunodeficiency virus (HIV). The extraction process is crucial for obtaining pure nucleic acids in an undegraded form, which is necessary for accurate analysis and experimentation. By extracting RNA from transfected cells, researchers can separate non-genomic nucleic acids from genomic nucleic acids, enabling the study of specific target nucleic acids without the need for cell lysis or other harsh methods. Overall, RNA extraction from transfected cells is essential for understanding viral replication, developing vaccines, and conducting accurate genetic analysis.
Why is RNA extracted from transfected cells for genetic studies?5 answersRNA is extracted from transfected cells for genetic studies because it allows for the investigation of the phenotypic consequences of gene suppression. This is done using RNA-transfected cell microarrays, where siRNA complexed with transfection reagent is printed on glass slides to form arrays of transfected cells. The extracted RNA can be used in a variety of reactions, such as reverse transcription and PCR, to study gene expression and function. Additionally, successful extraction of RNA is crucial for obtaining pure nucleic acids in an undegraded form. A selective extraction process is required to remove unwanted cellular material and minimize degradation of the RNA. This can be achieved through cell homogenization in an aqueous medium containing a strong detergent and a chelating agent, followed by selective extraction with phenol and precipitation of the RNA from the aqueous phase.
Why are RNA samples prone to contamination?5 answersRNA samples are prone to contamination due to various factors. One major source of contamination is the presence of genomic DNA (gDNA) in RNA preparations, which can generate false results in RNA-seq studies. Another source of contamination is off-target RNA contamination, which can occur during whole transcriptome sequencing experiments. Additionally, in single-cell RNA-seq experiments, ambient contamination can occur due to nucleic acid materials released by dead cells into the loading buffer. Reagents and cross-contamination across samples can also contribute to contamination in molecular biology laboratories. These contaminations can lead to inaccurate results and affect the quantification of transcripts, the detection of novel gene elements, and the quality of scRNA-seq data. Therefore, it is important to be cautious when interpreting results associated with low-abundance transcripts and to implement strategies to detect and remove contamination in RNA samples.
Why RNA extraction is tougher than DNA extraction?3 answersRNA extraction is tougher than DNA extraction due to several factors. RNA is a fragile molecule that requires clean conditions and careful handling, compared to the more rugged DNA. RNA is also transient and easily degraded by RNase enzymes, which are present for biological cellular function. Additionally, the extraction of high-quality RNA requires the removal of proteins and inhibitor enzymes that can interfere with downstream processes. Furthermore, the long-term survival of RNA in postmortem tissues is challenging, with high rates of null/inconclusive PCR-based results observed in aged/forensic specimens. Despite these challenges, RNA extraction is crucial for understanding the various types and functions of RNA, which play important roles in cellular processes and disease development.
What is not a difference between DNA and RNA?5 answersDNA and RNA have several differences, but one thing that is not a difference between them is the presence of adenine, guanine, and cytosine in both DNA and RNA.
Is extracted RNA infectious?10 answers