Why is there no bands on an RT-PCR mixture?
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There can be several reasons why there are no bands on an RT-PCR mixture. One possible reason is the presence of pseudogenes, which are nonfunctional genes or gene duplicates that can interfere with the amplification process . Another reason could be the use of total RNA instead of mRNA, as non-specific bands can be formed when amplifying total RNA . Additionally, the quality of primer design and the calibration of amplification conditions, such as the mixture and temperature, can also affect the presence of bands in the RT-PCR . It is important to note that the detection system used to identify the amplified targets must be specific to ensure accurate results . Finally, if the RT-PCR is performed on dried or nondried gels, using a transparency film as a mask can help recover the bands of interest .
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