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Yield of cell disruption of c. vulgaris using bead mill? in percentage? 


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The yield of cell disruption of Chlorella vulgaris using a bead mill was found to be 17.6% for chloroform–methanol solvent combination and 13.9% for hexane–ethanol solvent combination . Additionally, a study on the release kinetics of pDNA in a bead mill showed that the optimized conditions for pDNA(t) yield were 13.26 mg/g dcw (93.41% recovery) and for pDNA(sc) yield were 7.65 mg/g dcw (92.05% recovery) . Furthermore, a comparative evaluation of different cell disruption methods for lipid extraction from marine C. vulgaris cells revealed that bead milling resulted in a lipid concentration of 29% . These findings highlight the efficiency of bead milling as a cell disruption method for extracting lipids from Chlorella vulgaris, with yields ranging from 13.9% to 29%.

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Book ChapterDOI
Horst Schütte, Maria-Regina Kula 
26 Aug 2020
3 Citations
The paper does not specify the yield of cell disruption for C. vulgaris using a bead mill in percentage. Not addressed in the paper.
Bead milling method yielded 10% of lipids from Chlorella vulgaris cells.
Bead Mill method for Chlorella vulgaris cell disruption yielded 26.1% lipid content, as per the study.
Bead mill method yielded 15.2% lipid extraction from Chlorella vulgaris. It was one of the selected and optimized cell wall disruption methods in the study.

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What are the disadvantages of using bead mill to cell disruption of microalgae?5 answersThe disadvantages of using bead milling for cell disruption of microalgae include high energy consumption and operating costs compared to other methods. Bead milling can result in inefficient disruption of certain microalgae strains, such as Tetraselmis suecica, leading to lower lipid extraction yields. Additionally, bead milling may not be as effective in releasing specific biochemical compounds, like phenolic compounds, when compared to other disruption methods. Moreover, bead milling can subject microalgae cells to higher shear rates than alternative methods, potentially impacting the integrity of sensitive intracellular components. Overall, while bead milling is efficient in some aspects of cell disruption, its drawbacks in terms of energy consumption, effectiveness on different microalgae strains, and impact on specific biochemical compounds should be considered when choosing a disruption method.
Time of operation of bead mill equipment for microalgae c.vulgaris at industrial scale?5 answersThe time of operation of bead mill equipment for microalgae C. vulgaris at an industrial scale can vary based on the specific equipment and processing parameters. Studies have shown that the degree of cell disruption and the efficiency of extraction increase with factors such as the bead diameter, agitator speed, and the number of passes through the chamber. Additionally, the addition of a polar co-solvent like ethanol can significantly enhance the total extract yield of lipids, chlorophyll, and carotenoids during bead milling processes. Furthermore, alternative cell disruption methods like pulsed electric field (PEF) treatment have demonstrated the potential to improve extraction yields of valuable compounds from microalgae, showcasing the importance of exploring various techniques for efficient extraction at an industrial scale.
Bead mill recovery for microalgae cell lysis percentage?5 answersBead milling has been extensively studied for efficient cell disruption in microalgae. Studies have shown that the efficiency of cell lysis using bead mills can vary significantly depending on the microalgae species and the specific conditions applied. For instance, in the case of Tetraselmis chui, differences in physiological states of the microalgae significantly impacted cell disruption and protein recovery yields, highlighting the importance of understanding the biological state of the microalgae prior to processing. Additionally, optimization studies on Nannochloropsis oceanica, Nannochloropsis gaditana, and Tetraselmis suecica revealed that bead milling was effective in extracting lipids from certain strains, with specific energy consumption being a crucial factor in process selection. Furthermore, continuous bead milling processes have been developed to enhance intracellular protein recovery, showcasing the potential for maximizing recovery efficiency in a scalable and economical manner.
How can rupture bacteria with disruptor bead?5 answersBacteria can be ruptured using disruptor beads through various methods. One effective technique involves mechanical disruption using small glass beads in the presence of agitation. This method has been shown to be successful for a range of bacterial genera including Streptococcus, Lactobacillus, Staphylococcus, Bacillus, and Escherichia. Additionally, microfluidic chips with electroporation techniques have been utilized for Salmonella cell disruption, where a gold electrode array on a glass substrate is used to create an electric field that ruptures the bacterial cells. Furthermore, high-speed bead mills have been employed for the disruption of bakers' yeast, demonstrating the release of soluble proteins through disruption kinetics following a first-order rate equation. These methods showcase diverse approaches to effectively rupture bacteria using disruptor beads.
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