Showing papers on "Arabitol published in 2016"
TL;DR: In this paper, the authors used supported ruthenium and platinum bimetallic catalysts to convert lignocellulosic biomass of Japanese cedar, eucalyptus, bagasse, empty fruit bunch, and rice straw.
50 citations
TL;DR: The potential for BBV arrays as an attractive alternate to HPLC to analyze mixtures of sugars and sugar alcohols in biomedical applications is demonstrated and light is shed on structural motifs that make this possible.
Abstract: With the aim of discerning between different sugar and sugar alcohols of biomedical relevance, such as gut permeability, arrays of 2-component probes were assembled with up to six boronic acid-appended viologens (BBVs): 4,4'-o-BBV, 3,3'-o-BBV, 3,4'-o-BBV, 4,4'-o,m-BBV, 4,7'-o-PBBV, and pBoB, each coupled to the fluorophore 8-hydroxypyrene, 1,3,6-trisulfonic acid trisodium salt (HPTS). These probes were screened for their ability to discriminate between lactulose, l-rhamnose, 3-O-methyl-d-glucose, and xylose. Binding studies of sugar alcohols mannitol, sorbitol, erythritol, adonitol, arabitol, galactitol, and xylitol revealed that diols containing threo-1,2-diol units have higher affinity for BBVs relative diols containing erythro-1,2 units. Those containing both threo-1,2- and 1,3-syn diol motifs showed high affinity for boronic acid binding. Fluorescence from the arrays were examined by principle component analysis (PCA) and linear discriminant analysis (LDA). Arrays with only three BBVs sufficed to discriminate between sugars (e.g., lactulose) and sugar alcohols (e.g., mannitol), establishing a differential probe. Compared with 4,4'-o-BBV, 2-fold reductions in lower limits of detection (LOD) and quantification (LOQ) were achieved for lactulose with 4,7-o-PBBV (LOD 41 μM, LOQ 72 μM). Using a combination of 4,4'-o-BBV, 4,7-o-PBBV, and pBoB, LDA statistically segregated lactulose/mannitol (L/M) ratios from 0.1 to 0.5, consistent with values encountered in small intestinal permeability tests. Another triad containing 3,3'-o-BBV, 4,4'-o-BBV, and 4,7-o-PBBV also discerned similar L/M ratios. This proof-of-concept demonstrates the potential for BBV arrays as an attractive alternate to HPLC to analyze mixtures of sugars and sugar alcohols in biomedical applications and sheds light on structural motifs that make this possible.
17 citations
TL;DR: It is concluded that this route by plasmid-free E. coli has potential to become a commercially viable process for xylitol production.
Abstract: High costs and low production efficiency are a serious constraint to bio-based xylitol production. For industrial-scale production of xylitol, a plasmid-free Escherichia coli for arabitol-free xylitol production from corncob hemicellulosic hydrolysate has been constructed. Instead of being plasmid and inducer dependent, this strain relied on multiple-copy integration of xylose reductase (XR) genes into the chromosome, where their expression was controlled by the constitutive promoter P43. In addition, to minimize the flux from L-arabinose to arabitol, two strategies including low XR total activity and high selectivity of XR has been adopted. Arabitol was significantly decreased using plasmid-free strain which had lower XR total activity and an eight point-mutations of XR with a 27-fold lower enzyme activity toward L-arabinose was achieved. The plasmid-free strain in conjunction with this mutant XR can completely eliminate arabitol formation in xylitol production. In fed-batch fermentation, this plasmid-free strain produced 143.8 g L−1 xylitol at 1.84 g L−1 h−1 from corncob hemicellulosic hydrolysate. From these results, we conclude that this route by plasmid-free E. coli has potential to become a commercially viable process for xylitol production.
14 citations
TL;DR: The capabilities demonstrated here suggest that the DART-MS method, in conjunction with size-exclusion HPLC, is an effective approach for the analysis of saccharide biomarkers in fungi.
Abstract: Direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS) and size-exclusion HPLC – DRI are used, respectively, to qualitatively and quantitatively determine the carbohydrates extracted from the corn rot fungus Fusarium verticillioides. In situ permethylation in the DART beam forms tri-methylammonium adducts of the carbohydrates, which enables positive ion MS detection and analysis of these compounds in either whole fungal extracts or size-exclusion separated HPLC fractions of the extracts. This method detects the disaccharide trehalose at concentrations greater than 3.0 ppm. Additionally, this procedure allows for analysis of glucose as well as the sugar alcohols mannitol, arabitol, and glycerol. The reliability of the DART-MS method is confirmed by the absence of trehalose in a mutant strain of F. verticillioides lacking the gene for trehalose-6-phosphate synthase. The capabilities demonstrated here suggest that the DART-MS method, in conjunction with size-exclusion HPLC, is an effective approach for the analysis of saccharide biomarkers in fungi.
7 citations
01 Jan 2016
3 citations
Patent•
05 Feb 2016
TL;DR: In this article, a nutraceutical formulation of Curcumin and molecular complex former selected from Trehalose, Sucralose, Arabitol, Xylitol, Citric Acid, Carnosine, Maltose, Maltitol and mixture thereof is disclosed.
Abstract: A nutraceutical formulation of Curcumin and molecular complex former selected from Trehalose, Sucralose, Arabitol, Xylitol, Citric Acid, Carnosine, Maltose, Maltitol and mixture thereof is disclosed. A nutraceutical formulation comprises a molecular complex of Curcumin and molecular complex former selected from Trehalose, Sucralose, Arabitol, Xylitol, Citric Acid, Carnosine, Maltose, Maltitol and mixture thereof. The molecular complex is having improved water solubility and improved bioavailability.
1 citations
01 Jan 2016
TL;DR: Cultures of Dendryphiella salina were transferred to a similar but more dilute medium until the mycelial soluble carbohydrates had been depleted and so-called starved cultures were placed in various defined media, differing particularly with respect to concentrations of glucose and nitrate.
Abstract: SUMMARY Cultures of Dendryphiella salina, grown in a liquid glucose-tryptone medium, were transferred to a similar but more dilute medium until the mycelial soluble carbohydrates had been depleted. These so-called starved cultures were then placed in various defined (regrowth) media, differing particularly with respect to concentrations of glucose and nitrate. The changes in mycelial carbohydrates, which occurred during starvation, and afterwards, in the regrowth media, were determined by gas chromatography. Mannitol and arabitol were the major products of glucose assimilation. When this sugar was depleted from the medium, mannitol was readily catabolized. By contrast, accumulation of arabitol continued for several hours after the exhaustion of substrate glucose and the maximum levels attained were dependent upon the nitrogen source. Microscopical observations on the mycelium during starvation and regrowth are reported. The results are discussed in relation to other studies on the growth of fungi in stirred liquid culture.
Patent•
15 Jun 2016
TL;DR: In this paper, a xylose reductase mutant, genetically engineered bacteria and application in producing xylitol was revealed, and the amino acid sequence of the mutant was shown in the SEQ ID No 1.
Abstract: The invention discloses a xylose reductase mutant, genetically engineered bacteria and application in producing xylitol The amino acid sequence of the xylose reductase mutant is shown in the SEQ ID No1 Mutation of eight amino acid sites is carried out on xylose reductase, the activity of the xylose reductase mutant for catalyzing arabinose into arabitol is lowered, the selectivity of the xylose reductase mutant for converting xylitol is improved, and the by-product arabitol in the bioconversion product is not detected The separation step of xylitol bioconversion production downstream can be simplified, the production cost is reduced, and the purity of the xylitol product is improved