Showing papers on "Chemical library published in 2001"

Towards the development of molecularly imprinted artificial receptors for the screening of estrogenic chemicals

Abstract: Molecularly imprinted polymers are prepared using various steroid compounds as the templates. The imprinted polymers can selectively re-bind the original print molecules, which leads to versatile potential applications. The feasibility of using these artificial receptors to replace their biological counterparts for preliminary screening of a chemical library is demonstrated. A steroid library composed of 22 closely related compounds is screened with an estrogen specific polymer. The print molecule is identified with accuracy and structural similarities of other members are correlated with normalized retention indices. Molecularly imprinted artificial receptors are envisioned as being useful for screening purposes in drug discovery or for identifying endocrine-disrupting chemicals.

The cloning of human genes using cDNA phage display and small-molecule chemical probes.

Abstract: The cloning of genes based on protein function has become a powerful tool for protein discovery and should play an important role in proteomics in general. We have recently reported a technique for the functional identification of protein targets by combining traditional affinity chromatography with cDNA phage display. This procedure, referred to as display cloning, directly couples biologically active natural products to the gene of their protein cellular target. We now report the cloning of a human gene, the domain of F1 ATP synthase, using a synthetic scaffold molecule which serves as a prototype for a diverse chemical library. The ability to select genes from cDNA libraries using probes from combinatorial libraries would greatly increase the number of small molecule/protein interactions that can be identified. This method might prove valuable in furthering our understanding of biology and its application toward drug development.

Method for determining the structure of an active member of a chemical library

Abstract: A method for determining the structure of an active member of a chemical library is disclosed.

Method and compositions for drug discovery

Abstract: A drug-binding substrate formed or identified using a drug substance having a predetermined biological activity is used to screen and identify test compounds likely to exhibit the predetermined biological activity. The potential biologically active test compounds are identified by their specific binding to the drug-binding substrates as detected by any of a wide variety of techniques using labeled or unlabeled assay components. In one embodiment a monoclonal antibody raised against a drug substance is used as a drug-binding substrate to identify and isolate test compounds in a natural product extract or a combinatorial chemical library. Preferably the monoclonal antibody is characterized by its ability to bind specifically to at least one other drug substance having the same or similar biological activity as the drug substance against which it was raised. The invention finds use inter alia in drug discovery protocols, in toxicity profiling of drug substances and in assaying commercial natural products.

Radiofrequency encoded chemical library synthesis particles method

Abstract: A radiofrequency encoded chemical library synthesis particle which comprises a read-only radiofrequency tag linked to a solid phase. Chemical libraries synthesised on such particles and their use in biological screening methods.

Parallel chemical reactor

Abstract: The present invention relates to chemical libraries for identifying receptor-ligand interactions. It further provides for coding and reading individual library supports for structure indentification of the attached chemical entity. Moreover, the present invention provides a method for identifying a molecule of interest in a biological or chemical library.

Identification of a Potent and Selective Oxytocin Antagonist, from Screening a Fully Encoded Differential Release Combinatorial Chemical Library.

Abstract: A library of 1,296 1,4-benzodiazepines was prepared on 160 microM Tentagel beads. Compounds are attached to the beads using orthogonally cleavable linkers. The library was first screened as pools of 30 beads where 50% of the material is released and screened. GW405212X, a selective oxytocin antagonist, was identified by picking single beads from active pools.