Showing papers on "Chitinase published in 1970"
TL;DR: From observations, the co-axial distribution of polymers such as β1, 3-3, 6-glucans, glycoproteins, proteins, chitin and cellulose in the walls of each species is inferred, which has both similarities and differences between the species.
Abstract: SUMMARY: The appearance of the walls of apical and sub-apical regions of hyphae of predominantly 5 day cultures of Neurospora crassa, Schizophyllum commune and Phytophthora parasitica as seen with the electron microscope, employing shadowed or sectioned material, is illustrated and described in detail. The appearance of untreated or control material in buffer is compared with that exposed to various single and sequential treatments with enzymes, including laminarinase, Pronase, cellulase and chitinase, as well as various chemical treatments.
From these observations is inferred the co-axial distribution of polymers such as β1, 3-, β1, 6-glucans, glycoproteins, proteins, chitin and cellulose in the walls of each species. The distributions have both similarities and differences between the species. The significance of all these features for the growth, mechanical rigidity and integrity of a hypha is briefly discussed.
204 citations
TL;DR: Melanin-bound chitin was extremely resistant to enzymic degradation and substrate protection by melanin in the context of its antimycolytic properties have been discussed.
168 citations
TL;DR: The autolysis of mature fruiting bodies of Coprinus lagopus is caused by the degradation of cell walls, accomplished by the action of chitinases which are newly formed shortly before spore release begins.
Abstract: Surmmary
The autolysis of mature fruiting bodies of Coprinus lagopus is caused by the degradation of cell walls. This process is accomplished by the action of chitinases which are newly formed shortly before spore release begins. Chitinase activity is localized intracellularly in vacuoles together with other hydrplytic enzymes. It is released into the wall upon cessation of metabolic activity in senescing gills.
89 citations
TL;DR: Comparisons with alpha-(1 --> 3)-glucan isolated from Aspergillus niger and Polyporus betulinus and with chitin from fungal origin support the point of view that short and thick fibers were observed on the outer surface of the yeast phase of Paracoccidioides brasiliensis.
Abstract: Short and thick fibers were observed on the outer surface of the yeast phase of Paracoccidioides brasiliensis, and long and thin fibers were seen on the inner surface The long fibers disappear with chitinase treatment and are composed of chitin The short fibers disappear under alkali treatment and are composed of α-glucan Comparisons with α-(1 → 3)-glucan isolated from Aspergillus niger and Polyporus betulinus and with chitin from fungal origin support our point of view
65 citations
Journal Article•
TL;DR: Streptomyces sp chitinase purification and properties by column chromatography, noting calcium component is noted.
Abstract: Streptomyces sp chitinase purification and properties by column chromatography, noting calcium component
45 citations
TL;DR: In this article, a strain of the entomophagous fungus Beauveria bassiana was cultivated under stationary and submersed conditions, and the presence of chitinase, cellulase, proteases, and lipase was ascertained.
42 citations
TL;DR: Mode of lysis of cell walls of P. oryzae P2 by a single and combined action of lytic enzymes were compared and Combination of β-1→3-glucanase and chitinase gave the most rapid and extensive lysis.
Abstract: Cell walls were prepared from Piricularia oryzae P2. Lytic enzymes were produced when Bacillus circulans WL 12 was grown with P. oryzae cell wall as a sole source of carbon. Mode of lysis of P. oryzae cell walls was compared with the lysis of walls of Aspergillus oryzae and Neurospora crassa. Activities of β-1→3-glucanase, chitinase, and β-1→6-glucanase were demonstrated in the culture filtrate. Modes of lysis of cell walls of P. oryzae P2 by a single and combined action of lytic enzymes were compared. Combination of β-1→3-glucanase and chitinase gave the most rapid and extensive lysis.
30 citations
1 citations