Showing papers on "Genomics published in 1991"
TL;DR: Advances in the manipulation and analysis of large DNA fragments have made it possible to develop detailed molecular maps of the Drosophila genome as the initial steps in determining the complete DNA sequence.
Abstract: An ultimate goal of Drosophila genetics is to identify and define the functions of all the genes in the organism. Traditional approaches based on the isolation of mutant genes have been extraordinary fruitful. Recent advances in the manipulation and analysis of large DNA fragments have made it possible to develop detailed molecular maps of the Drosophila genome as the initial steps in determining the complete DNA sequence.
69 citations
TL;DR: It is argued that the HGP is a new form of coordinated, interdisciplinary science; its primary objective must be seen as the creation of a tool for bio‐medical research — a source book that will be the basis of study of variation and function for a long time.
Abstract: The human is estimated to have at least 50,000 expressed genes (gene loci) Some information is available concerning about 5000 of these gene loci and about 1900 have been mapped, ie, assigned to specific chromosomes (and in most instances particular chromosome regions) Progress has been achieved by a combination of physical mapping (eg, study of somatic cell hybrids and chromosomal in situ hybridization) and genetic mapping (eg, genetic linkage studies) New methods for both physical and genetic mapping are expanding the armamentarium The usefulness of the mapping information is already evident; the spin-off from the Human Genome Project (HGP) begins immediately The complete nucleotide sequence is the ultimate map of the human genome Sequencing, although already under way for limited segments of the genome, will await further progress in gene mapping, and in particular creation of contig maps for each chromosome Meanwhile the technology of sequencing and sequence information handling will be developed It is argued that the HGP is a new form of coordinated, interdisciplinary science; that its primary objective must be seen as the creation of a tool for biomedical research--a source book that will be the basis of study of variation and function for a long time; that the impact on scientist training will be salutary by relieving graduate students of useless drudgery and by training scientists competent in both molecular genetics and computational science; and that the funding of the HGP will have an insignificant negative effect on science funding generally, and indeed may have a beneficial effect through economy of scale and a focusing of attention on the excitement of biology and medical science
66 citations
TL;DR: An automated DNA sequencing system, the collaborative effort of Japanese scientists and engineers, which has a potential output of up to 108,000 bases per day is described.
Abstract: An automated DNA sequencing system, the collaborative effort of Japanese scientists and engineers, which has a potential output of up to 108,000 bases per day is described.
15 citations
TL;DR: The European-Community-funded Bioteehnology Action Programme (BEAP) was a 2-year project to determine the sequence of the entire chromosome of a eukaryotic cell as discussed by the authors.
7 citations
TL;DR: The evolution of the chloroplast genome and the endosymbiont hypothesis are related to the results and those of others which show the occurrence of cpDNA sequences common to the nuclear and chlorOPlast genome.
Abstract: The role of chloroplast (cp) DNA in plastid and chloroplast function is discussed, particularly in relation to the interaction with nuclear DNA. The evolution of the chloroplast genome and the endosymbiont hypothesis are related to our results and those of others which show the occurrence of cpDNA sequences common to the nuclear and chloroplast genome.
4 citations
31 Oct 1991