Showing papers on "Gentiana published in 2005"

Somatic embryogenesis of Gentiana genus IV.: Characterisation of Gentiana cruciata and Gentiana tibetica embryogenic cell suspensions

Abstract: Experiments to characterise long-term embryogenic suspension cultures of Gentiana cruciata (L.) and G. tibetica (King) are reported. Cell suspensions of both species differed in the percentage of five selected fractions of cell aggregates, as well as in fresh and dry mass during three years of culture. In G. tibetica the ratio of cells in phase G2 to G1 was higher than in G. cruciata. The response of suspension cultures to GA3 (at 0, 1.49 or 2.89 µmol), kinetin (at 0, 2.32, 4.64 or 9.28 µmol) and adenine sulphate (at 0 or 434 µmol) was studied. The increase of kinetin concentration stimulated embryo production in suspensions of G. tibetica. Somatic embryo production in G. tibetica was significantly higher than in G. cruciata. In G. tibetica, the aggregate fraction >450 µm was at least four times more productive than the same fraction in G. cruciata suspensions.

Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi

Abstract: Objective To in vestigate the constituents in root of Gentiana macrophylla. Method Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.) and identified by comparing with standard substance. Result Eight compounds were identified. Four compounds isolated from the chloroform fraction are: 5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one (1), erythrocentauric acid (2), roburic acid (3), oleanolic acid (4). Water fraction gave four known secoiridoid glucosides. They were: gentiopicroside (5), swertiamarine (6), sweroside (7), 6'-O-beta-D-glucosylgentiopicroside (8). Conclusion 1 is a novel compound. It was named as erythrocentauric acid. 2 was isolated from genus Gentiana and 8 was isolated from G. macrophylla for the first time.

Use Of Plants, Plant Extracts And Nature-Identical Components From Plants To Affect The Rumen Fermentation And To Improve The Energy And Protein Retention Of Ruminants

Abstract: Use of an plant materials, plant extracts or nature-identical components selected from Lonicera japonica, Gentiana asclepidea, Gentiana lutea, Eugenia caryophyllata, Bellis perennis, Olea europaea, Symphytum officinale, Carduus pycnocephalus, Paeoniae alba radix, Populus tremula, Prunus avium, Salix caprea, Rheum nobile, Helianthemum canum, Arctostaphylos uva-ursi, Peltiphyllum peltatum, Epilobium montanum, Knautia arvensis, Latuca sativa and Urtica dioica and extracts thereof, and b-myrcene to affect the rumen fermentation and to increase the availability of energy and nitrogen source for a ruminant.

Phylogeny of Metagentiana and related genera (Gentianaceae) inferred from nuclear ribosomal ITS sequences

Abstract: The infrageneric phylogeny of Metagentiana, a newly erected genus in the Gentianaceae, was constructed based on the analysis of the nucleotide sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA of 10 representative species of this genus and 22 species of Gentiana, Tripterospermum and Crawfurdia. The analysis indicates that the genus Metagentiana is polyphyletic, because all sampled species of Tripterospermum and Crawfurdia are clearly embedded within Metagentiana. The species of Tripterospermum and Crawfurdia also did not cluster respectively as two monophyletic clades. These results do not merit status of three separate genera. However, these three genera clustered together as a monophyletic clade sister to Gentiana, which is in agreement with the previous conclusions on the separation of Metagentiana from Gentiana and its closer relationship to Tripterospermum and Crawfurdia based on studies of gross morphology, floral anatomy, chromosomes, palynology and embryology. The ITS sequences do not provide sufficient information to make a robust estimation of the phylogenetic relationships among dysploid cytotypes of x=17, 21 and 23, within the Metagentiana, Tripterospermum and Crawfurdia complex, but the preliminary results seem to indicate their reticulate and paralleling evolution.

Use of plants and plant extracts as feed additive to affect the rumen fermentation

Abstract: Use of an plant materials, plant extracts or nature-identical components selected from Lonicera japonica, Gentiana asclepidea, Gentiana lutea, Eugenia caryophyllata, Bellis perennis, Olea europaea, Symphytum officinale, Carduus pycnocephalus, Paeoniae alba radix, Populus tremula, Prunus avium, Salix caprea, Rheum nobile, Helianthemum canum, Arctostaphylos uva-ursi, Peltiphyllum peltatum, Epilobium montanum, Knautia arvensis, Latuca sativa and Urtica dioica and extracts thereof, and β-myrcene to affect the rumen fermentation and to increase the availability of energy and nitrogen source for a ruminant.

Study on the chemical constituents of Gentiana scabra Bge

Abstract: Objective To study the chemical constituents of Gentiana scabra BgeMethods Six compounds were isolated by the silica gel and sephadex LH-20 column chromatography,and identified by the physico-chemical characters and many kinds of spectroscopic analysesResults Six compounds were isolated from the EtOAc extract of Gentiana scabra BgeThey are separately kaempferol,L-sesamin,salicylic acid,ferulic acid,oleanolic acid and pranferinConclusion Among them, pranferin is separated from Gentiana for the first time

Anticancer agent containing extract of gentiana spp, health assistant food, cosmetic for medicinal use, and method for producing extract of gentiana spp

Abstract: PROBLEM TO BE SOLVED: To effectively utilize a root and a leaf of Gentiana triflora which are conventionally discarded. SOLUTION: The anticancer agent as an oral medicine or an external preparation contains a crude extract of a plant of Gentiana spp, or a purified product obtained therefrom as an active ingredient. The Gentiana triflora is preferably used as the Gentiana spp. The root or the leaf, especially the root is preferably used as the part of the plant. The crude extract or the purified product can be used as the health assistant food (supplement) and the cosmetic for medicinal use. COPYRIGHT: (C)2007,JPO&INPIT

Fat-soluble constituents in roots of Gentiana macrophylla from Shaanxi Province

Abstract: Objective To systematically study the chemical constituents in the roots of Gentiana macrophylla from Shaanxi Province. Methods Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, (~1H-NMR), (~(13)C-NMR), (~1H-~1H COSY) and (~1H-~(13)C COSY), HMBC, and HMQC) and identified by comparing with the authentic substance. Results Five compounds were got from fat-soluble fraction. They are identified as: N-pentacosyl-2-carboxy-benzoyl amide (Ⅰ), 5-carboxyl-3, 4-dihydrogen-1H-2-benzopyran-1-one (Ⅱ), erythrocentaurin (Ⅲ), roburic acid (Ⅳ), oleanolic acid (Ⅴ). Conclusion Compounds Ⅰ and Ⅱ are two novel ones. They are named as qinjiaoamide and erythrocentauric acid, respectively. Compound Ⅲ is isolated from the plants of Gentiana L. for the first time.