Showing papers on "Glucose Measurement published in 1986"
TL;DR: The main blood constituents which could interfere in clinical glucose measurements using a hydrogen peroxide based glucose electrode have been investigated using several different membranes and constant and sweeping potentials.
147 citations
TL;DR: In this article, an enzyme electrodes was used for glucose determination in unstirred, undiluted whole blood, and the system comprises an H2O2-detecting electrode upon which is placed a membrane laminate incorporating glucose oxidase.
105 citations
TL;DR: Comparison of Type II with Type I analyses revealed a possible explanation for discrepancies in the estimates of nonsaturable glucose transfer by different methods and highlighted the importance of tissue glucose measurements in studies of unidirectional glucose influx.
Abstract: Regional blood-brain glucose transfer was studied in pentobarbitone-anaesthetized rats using a programmed intravenous infusion technique that maintained steady levels of unlabeled (up to 55 mM) and tracer D-glucose in the circulating plasma. Regional cerebral blood flow, glucose phosphorylation rate, and tissue glucose content were also measured under comparable conditions. Data were analysed in terms of irreversible Michaelis-Menten kinetics assuming independent influx and efflux (Type I) and reversible Michaelis-Menten kinetics (Type II) across both the luminal and the abluminal membranes of the endothelial cell. The latter analysis corresponds to simple stereospecific membrane pores. The mathematical model allowed for changes in tissue glucose content and back-diffusion of tracer during the experiments. Type I analyses gave Kt values of approximately 6.6 mM, whereas those by Type II were consistently lower. Interregional differences were not significant using either scheme. Comparison of Type II with Type I analyses revealed a possible explanation for discrepancies in the estimates of nonsaturable glucose transfer by different methods and highlighted the importance of tissue glucose measurements in studies of unidirectional glucose influx. Since the experimental data may be described equally well by either scheme and some interaction between influx and efflux across the endothelial cell might be expected, consideration of this alternative approach is suggested.
62 citations
TL;DR: Computer-assisted insulin dose decision-making is feasible, safe, and effective in enabling persons with type I diabetes mellitus to achieve lower mean blood glucose values over a six-week period while initiating pump therapy.
33 citations
TL;DR: Capillary glucose monitoring can provide a valuable adjunct to the care of hospitalized patients with diabetes and was initiated for instructing general staff nurses in the use of Chemstrips bG strips and the Accu-Chek bG meter.
26 citations
TL;DR: Improvement of the electrocatalytic method of glucose determination was achieved by applying the pulse voltammetry technique which enhances selectivity of a platinum-black working electrode for glucose measurement and diminishes interference by low molecular weight serum components such as serum urea.
Abstract: Improvement of the electrocatalytic method of glucose determination was achieved by applying the pulse voltammetry technique. This technique, compared. to previously applied cyclic voltammetry, enhances selectivity of a platinum-black working electrode for glucose measurement. A low catalytic load working electrode has been used which is less susceptible to poisoning and has longer term stability. With cyclic voltammetry previously applied, those electrodes were limited by their narrow concentration range for glucose detection. By applying a pulsing technique, these electrodes exhibit linearity up to a glucose concentration 200 mg/dl with correlation coefficients greater than 0.9. The pulse voltammetry diminishes interference by low molecular weight serum components such as serum urea. Concentrations from 20. to 40 mg/di had an interference of 4 percent compared to 40 percent with cyclic voltammetry. Further investigations are carried on to adopt pulse voltammetry for direct measurements of glucose in body fluids.
5 citations