Showing papers on "Mitochondrial DNA replication published in 1986"
TL;DR: A thermosensitive mutant which is transformed into a population of cells devoid of mitochondrial DNA (rho 0 cells) at 35 degrees C and is deficient in mitochondrial (mt) DNA polymerase activity is isolated, with no evidence that mip mutants are deficient in mtDNA repair.
62 citations
TL;DR: Fourteen temperature sensitive mutants affected in the maintenance of the mitochondrial (mt) genome have been isolated that display the following phenotype: growth on fermentable medium at 23°C and 35°C (exclusion of general temperature-sensitive mutants).
Abstract: This paper describes the isolation and characterization of mutants affected in the maintenance of the mitochondrial (mt) genome. The rationale of the screening procedure is based on the observation that the alkaloid lycorine inhibits growth of rho−-mutants, whereas rho°-mutants, devoid of mt DNA, are resistant to this drug (Del Giudice et al. 1984). Fourteen temperature sensitive mutants have been isolated that display the following phenotype:
-Growth on fermentable medium at 23°C and 35°C (exclusion of general temperature-sensitive mutants).
-no growth at 23°C and growth at 35°C on fermentable medium containing lycorine (selection for mutants producing rho°-petites).
-growth at 23°C and no growth an 35°C on non-fermentable medium (selection for temperature-dependent loss of respiratory competence).
6 citations