Showing papers in "Journal of Biological Chemistry in 1986"

TL;DR: The results demonstrate a functional involvement of fibronectin in mediating cellular responses to TGFbeta, and suggest a model for TGF beta action based on the control of the extracellular matrix in target cells.

TL;DR: Human brain tau and paired helical filament polypeptides co-migrated on sodium dodecyl sulfate-polyacrylamide gels suggest that tau is a major component of Alzheimer paired helicals filaments.

TL;DR: It is suggested that sphingosine will be a useful inhibitor for investigating the function of protein kinase C in vitro and in living cells.

TL;DR: Data are consistent with the K-ras protein functioning in transmembrane signalling by activating phospholipase C and Protein kinase C (Ca2+/phospholipid-dependent enzyme) activation by DAG may play an important role in cellular transformation.

TL;DR: Bone exhibits the most complex spectrum of growth factor activities of any tissue yet described, and mechanisms for unmasking or release of BDGFs from the mineralized matrix resulting in local action on target cells are undoubtedly important for the development and maintenance of bone tissue.

TL;DR: The browning of fFL incubation mixtures proceeded to a greater extent under a nitrogen versus an air atmosphere, suggesting that oxidative degradation of Amadori adducts to form CML may limit the browning reactions of glycated proteins.

TL;DR: The results indicate that brefeldin A is a unique agent which primarily impedes protein transport from the endoplasmic reticulum to the Golgi complex by a mechanism different from those considered for other secretion-blocking agents so far reported.

TL;DR: The overexpression of a similar anionic glutathione transferase in human breast cancer cells selected for multidrug resistance and in rat hyperplastic liver nodules, which develop resistance to various hepatotoxins, suggests a possible role for this drug-conjugating enzyme in the mechanism of resistance in both of these states.

TL;DR: The present report describes, for the first time, the identification of two constitutive forms of heme oxygenase, designated as HO-1 and HO-2, in rat liver microsomal fractions, and the two forms appeared as immunologically different entities.

TL;DR: The metabolism of the dihydropyridine calcium antagonist and vasodilator nifedipine has been reported to exhibit polymorphism among individual humans, and the amount of the P-450NF polypeptide may be a major factor in influencing the level of catalytic activity in humans as well as rats.

TL;DR: The results suggested two possible modes of action of ryanodine: 1) a change in the gating mechanism of the channel which is not readily detected using the slowly permeating molecule L-glucose or 2) achange in channel structure which prevents its complete closing.

TL;DR: It is concluded that diversification of preproglucagon gene expression occurs at the level of cell-specific post-translational processing.

TL;DR: Subcellular fractionation studies of homogenates of adrenal gland indicate that the recovery and enrichment of [3H]PK11195 binding sites in the nuclear, mitochondrial, microsomal, and soluble fractions correlate closely with cytochrome oxidase activity, but not with markers for the nuclei, lysosomes, peroxysomes, endoplasmic reticulum, plasma membrane, or cytoplasm.

TL;DR: It is possible that de-N-fatty acylation of gangliosides could be an effective means to modulate EGF receptor function in membranes.

TL;DR: The complete sequence of the cDNA clone representing the full size human skin collagenase mRNA is determined, and the primary structure of the coding region of the presented clone is homologous to an oncogene-induced rat protein whose function is still unknown, although preliminary observations suggest that it is not ratskin collagenase.

TL;DR: A unique cytochrome P-450-dependent fatty acid monooxygenase from Bacillus megaterium ATCC 14581 has now been purified to homogeneity from pentobarbital-induced bacteria as a single polypeptide and can catalyze the oxygenation of long chain fatty acids without the aid of any other protein.

TL;DR: The data suggest that the absolute ratio of Fe3+ to Fe2+ was the primary determining factor for the initiation of lipid peroxidation reactions.

TL;DR: Redox cycling of anthracyclines by mitochondrial NADH dehydrogenase is shown, to generate O2-, H2O2, and OH which may underlie the cardiotoxicity of these antitumor agents.

TL;DR: As an important new reagent for studying the cAMP-dependent protein kinase, a 20-residue peptide has been synthesized that corresponds to the active site of the skeletal muscle inhibitor protein.

TL;DR: Data show that CIF-A and TGF-beta are closely related or identical molecules and that these factors may be involved in cell differentiation including cartilage formation as the first step in endochondral bone formation.

TL;DR: It is suggested that anthrax lethal toxin requires passage through an acidic endocytic vesicle in order to exert its toxic effect within the cytosol.

TL;DR: Improved methods for the purification of ARF from bovine brain are described, and although the protein as purified contains stoichiometric amounts of GDP, GTPase activity of isolated ARF was not detected.

TL;DR: In this article, 32P-labeled complementary DNA probes encoding human and mouse Sm-C/IGF-I and human IGF-II were used in Northern blot hybridization to analyse rat SmC/I and insulin-like growth factor II mRNAs in poly(A+) RNAs from intestine, liver, lung, and brain of adults and fetal rats between day 14 and 17 of gestation.

TL;DR: It is shown that enzyme activities of procollagenase and prostromelysin are revealed after conditioned medium is analyzed by gel filtration chromatography or by electrophoresis on sodium dodecyl sulfate-substrate gels, suggesting that endogenous inhibitors regulate the expression of metalloproteinases secreted by endothelial cells.

TL;DR: In competitive assays, using unlabeled nucleic acid competitors, the DNA binding of Ku was not inhibited in the presence of yeast tRNA, synthetic copolymer of poly(A)-poly(dT), or circular plasmid pBR322 DNA, but was inhibited when the plasmids DNA was cleaved with appropriate restriction endonucleases.

TL;DR: The identical digestion patterns of reduced, carboxymethylated protein substrates indicated that both active forms of the enzyme have the same substrate specificity, which may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis.

TL;DR: The combined enzyme kinetic and concentration data showed that several of the inhibitors have the capacity to play physiologically important roles as cysteine proteinase inhibitors in many biological fluids.

TL;DR: It is concluded that the M1- and M2-type isozymes of pyruvate kinase are produced from the same gene probably by alternative RNA splicing.

TL;DR: The experiments suggest that injury to cardiac mitochondria which is produced by anthracycline antibiotics may result from the generation of the hydroxyl radical during anthrACYcline metabolism by NADH dehydrogenase.

TL;DR: Two areas of the precursor exhibit a marked degree of homology to the human counterpart and suggests an important biological function for this area of TGF-beta, which can act, often in cooperation with other growth factors, as a mitogenic factor for a variety of cells.