Showing papers on "Murashige and Skoog medium published in 1972"
TL;DR: A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium.
Abstract: A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussed
68 citations
TL;DR: These experiments were carried out to establish a method of anther culture of asparagus using Murashige and Skoog′s medium to clarify the effects of developmental stage of anothers, method of sterilization and concentration of growth regulating substances on callus and organ formation.
Abstract: These experiments were carried out to establish a method of anther culture of asparagus. Special efforts were made to clarify the effects of developmental stage of anthers, method of sterilization and concentration of growth regulating substances on callus and organ formation. The medium was prepared by adding 20g/l sucrose, 7.0g/l J-agar and growth regulating substances (N6-benzyladenine and NAA) to Murashige and Skoog′s medium (MS medium). The cultures were incubated at 25°C under 16 hours illumination (4, 000lux) a day with fluorescent lamps of daylight type. The experimental results are summarized as follows:
11 citations