Showing papers on "NSP1 published in 1991"
TL;DR: Two plasmids are constructed which under lac control expressed the SVLM21 and the SVToto forms, respectively, of the Sindbis virus nonstructural protein, nsP1, providing strong evidence that nsP 1 has methyltransferase activity in the absence of any other viral nonstructuring proteins.
115 citations
TL;DR: It is reported that the MPA-resistant phenotype of SVMPA has been mapped to the coding region for the nonstructural viral protein, nsP1, and results support the idea that an important function of nsP 1 relates to the modification of the 5' terminus of the Sindbis virus mRNAs.
112 citations
TL;DR: A mutation at nucleotide 1101 of Sindbis virus ts11 nsP1 caused temperature-sensitive negative-strand synthesis and suppressed the 24R phenotype, which was caused by a mutation in nsP4 as discussed by the authors.
Abstract: A mutation at nucleotide 1101 of Sindbis virus ts11 nsP1 caused temperature-sensitive negative-strand synthesis and suppressed the 24R phenotype, which is caused by a mutation in nsP4. Nonstructural proteins synthesized and accumulated by ts11 at 40 degrees C did not cause the reactivation of negative-strand synthesis upon return to 30 degrees C and did not prevent the formation of new replication complexes at 30 degrees C.
111 citations