Showing papers on "Paper chromatography published in 2012"
Journal Article•
TL;DR: In the course of preliminary experiments with amylases, phos-phorylases and phosphatases from various sources, it was found that some enzymes did not move at all while others moved considerable distances on the paper.
11 citations
TL;DR: In this article, the authors demonstrate how normal and reverse phase chromatography can be illustrated using only chromatography paper for the separation of extracts of dandelions, and show that it is possible to extract dandelion extracts from a single paper.
Abstract: This demonstration describes how normal and reverse phase chromatography can be illustrated using only chromatography paper for the separation of extracts of dandelions.
9 citations
TL;DR: The alkali-insoluble (1 → 3)-β-d-glucan was prepared by alkaline–acid method and followed deproteinization by alk alkaline method using yeast cell wall as the starting material and indicated that the alkaline-acid method was an ideal way for preparing glucan.
Abstract: The alkali-insoluble (1 → 3)-β-d-glucan was prepared by alkaline–acid method and followed deproteinization by alkaline method using yeast cell wall as the starting material. The deproteinized product was characterized by infrared (IR) spectroscopy, and the polysaccharide components were analysed by paper chromatography. It indicated that the alkaline–acid method was an ideal way for preparing (1 → 3)-β-d-glucan. The obtained glucan had high purity and good yield.
6 citations
01 Jan 2012
TL;DR: Chromatography and mass spectrometry were introduced as analytical methods used to identify drugs and controlled substances as mentioned in this paper, and the fundamental principles of paper chromatography, thin-layer chromatography and column chromatography were discussed.
Abstract: Chromatography and mass spectrometry are introduced as analytical methods used to identify drugs and controlled substances. The fundamental principles of paper chromatography, thin-layer chromatography (TLC), column chromatography (CC), ion-exchange chromatography (IEC), high-performance liquid chromatography (HPLC), and gas chromatography (GC) are discussed. Emphasis is on the use of mass selective detectors (MSDs) with gas chromatography, although several other detectors (flame ionization, thermal conductivity, electron capture, and nitrogen-phosphorus) are also described. The basic theory of mass spectrometry is presented including hard and soft ionization techniques, m/z determination, fragmentation patterns, types of mass analyzers (quadrupole, magnetic sector), and interpretation of spectra. Qualitative and quantitative aspects of chromatography and mass spectrometry are discussed, including interpretation of chromatograms, retention factors, retention times, normalized retention times, relative concentration, factors influencing chromatographic resolution, interpretation of MS spectra, base peaks, molecular ion peaks (M+), M + 1 peaks, and M + 2 peaks.
5 citations
Journal Article•
TL;DR: This study employed gold and silver nanoparticles to detect protein bands after the paper chromatography method, and noticed that location of the protein bands was clearly distinct and detectable with this technique.
Abstract: Paper chromatography is an analytical technique for separating and identifying mixtures of materials It is a useful technique since it is relatively quick, and requires small quantities of the material By emergence of nanotechnology, paper chromatography has found many applications in biology and biotechnology In this study, we employed gold and silver nanoparticles to detect protein bands after the paper chromatography method We first performed paper chromatography on a solution containing Bovine Serum Albumin (BSA) protein Then, the gold and silver nanoparticles were exploited for paper coloration As a result, it was noticed that location of the protein bands was clearly distinct and detectable with this technique
4 citations
TL;DR: In this article, an activating action of hexamethylenediamine is revealed in the copper (II)-catalyzed indicator reaction of iron (III) reduced by sodium thiosulfate.
Abstract: An activating action of hexamethylenediamine is revealed in the copper (II)-catalyzed indicator reaction of iron (III) reduced by sodium thiosulfate. It is shown that the addition of hexamethylenediamine to a mobile phase (thin-layer chromatography) and the impregnation of paper with hexamethylenediamine (paper chromatography) enhance the efficiency of metal separation for the model mixture containing Cu (II), Hg (II), Cd (II), and Pb (II). The techniques for the sorption-catalytic determination of copper (II) with the help of Sorbfil plates, including those in combination with thin-layer chromatography, are developed; they work within the concentration range from 0.005 to 10 μg/mL, the detection limit being 4 ng/mL, which is approximately an order of magnitude lower than in solution. The methods are tested in analysis of waste and river waters.
3 citations
Patent•
28 Apr 2012
TL;DR: In this paper, a sample pretreatment method based on a paper-based thin layer chromatography plate and an application of the method is described, which comprises the following steps of: loading a sample concentrated solution to be detected at the bottom of the paper based thin-layer Chromatography plate, putting the paper base into a developing tank, adding a developing agent for thin-layered chromatography separation, shearing a target detection substance stripe at a constant retardation factor value position, extracting a substance, thus completing sample pre-treatment.
Abstract: The invention discloses a sample pretreatment method based on a paper-based thin layer chromatography plate and an application of the method. The sample pretreatment method comprises the following steps of: loading a sample concentrated solution to be detected at the bottom of the paper-based thin layer chromatography plate, putting the paper-based thin layer chromatography plate into a developing tank, adding a developing agent for thin layer chromatography separation, shearing a target detection substance stripe at a constant retardation factor value position, extracting a substance to be detected, thus completing sample pretreatment. By the method, the stripe at the constant retardation factor value position of the component to be detected can be sheared and used for subsequent quantitative analysis, so that influence of interference substances at the rest positions is avoided, and the recovery rate is high; multiple paper-based thin layer chromatography separation can be simultaneously performed under the same developing condition, influence of condition difference on the separation result in the treatment process is avoided, and the method is good in parallelism; and the adopted paper-based thin layer chromatography plate is cheap and readily available, and the whole treatment method is easy to operate, environment-friendly, efficient, economical and practical.
TL;DR: The lactic acid bacteria to produce GABA and GABA determinations method were studied in this article, which suitable conditions for developing agent (N-butanol: acetic acid: water) volume ratio were 4: 1: 3, under 30 min, 80 °C, 510nm detection wavelength respectively.
Abstract: The lactic acid bacteria to produce GABA and GABA determinations method were studied. Research results show that the strains isolated belong to Lactobacillus delbruckii for GABA with morphological and physiological and biochemical characterization. Paper chromatography for determination of GABA, which suitable conditions for developing agent (N-butanol: acetic acid: water) volume ratio were 4: 1: 3, under 30 min, 80 °C, 510nm detection wavelength respectively.
01 Jan 2012
TL;DR: This article presents a review of basic chromatographic techniques applied to the determination of various pharmaceuticals and describes about the instruments, methods used in it.
Abstract: This article presents a review of basic chromatographic techniques applied to the determination of various pharmaceuticals is discussed. It describes about various Chromatographic techniques and is usage. Also it briefly describes about the instruments, methods used in it. Chromatographic separations can be carried out using a variety of supports, including immobilized silica on glass plates (thin layer chromatography), volatile gases (gas chromatography), paper (paper chromatography), and liquids which may incorporate hydrophilic, insoluble molecules (liquid chromatography).