Showing papers on "Sperm plasma membrane published in 1983"
TL;DR: The dose-response relationships indicate that inhibition of Ca2+ uptake does not bear a causal relationship to the activation of motility and metabolism and further suggest a common action of these agents rather than selective effects of D600 and TMB-8 upon Ca 2+ channels in the sperm plasma membrane.
51 citations
TL;DR: Comparison of the electrophoretic patterns of labelled sperm and of the membranes isolated from 125I-labelled sperm suggests that no major labelled proteins are lost during the isolation procedure, and so to this extent the membrane fraction is representative of the entire sperm plasma membrane.
Abstract: A subcellular fraction containing plasma membranes was isolated from flagella of the sperm of Strongylocentrotus purpuratus by differential centrifugation, and analysed by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. Coomassie Blue staining revealed nine major bands and 14 minor species. Five bands of apparent molecular weights approximately 200 X 10(3), 149 X 10(3), 120 X 10(3), 75 X 10(3) and 59 X 10(3) also stained with periodic acid-Schiff's reagent and so are probably glycoproteins. These five components are externally exposed, as determined by lactoperoxidase-catalysed radio-iodination. Isolation of membranes from radio-iodinated sperm results in an enrichment of about tenfold in the specific activity of 125I. Comparison of the electrophoretic patterns of labelled sperm and of the membranes isolated from 125I-labelled sperm suggests that no major labelled proteins are lost during the isolation procedure, and so to this extent the membrane fraction is representative of the entire sperm plasma membrane.
28 citations
TL;DR: Fertilizing capacity among uterine-capacitated rabbit sperm cells declined exponentially during incubation with membrane vesicles from seminal plasma, accompanied by diminished rates of vesicle uptake by spermatozoa and postbinding decapacitation half-times appear broadly alike.
21 citations
TL;DR: Energy-dispersive x-ray microanalysis suggests that sudden permeability changes in the sperm plasma membrane are associated with the acrosome reaction, but that complete breakdown of membrane and cell function does not occur for several minutes.
Abstract: Energy-dispersive x-ray microanalysis was used to analyze changes in the subcellular distributions of Na, Mg, P, S, Cl, K, and Ca associated with the acrosome reaction of sea urchin sperm. Within 5 sec after induction of the acrosome reaction, nuclear Na and mitochondrial Ca increased and nuclear and mitochondrial K decreased. Uptake of mitochondrial P was detected after several minutes, and increases in nuclear Mg were detected only after 5-10 min of incubation following induction of the reaction. The results suggest that sudden permeability changes in the sperm plasma membrane are associated with the acrosome reaction, but that complete breakdown of membrane and cell function does not occur for several minutes.
21 citations
TL;DR: Examination of freeze-fracture replicas of zebrafish spermatozoa with the transmission electron microscope has demonstrated an unique assemblage of intramembranous particles associated with a localized portion of plasma membrane.
16 citations
01 Jan 1983
TL;DR: It is the first two of these reactions in mouse gametes which the researchers have examining in their studies; this paper presents a brief account of the findings and interpretations.
Abstract: In the dance of sperm and egg which leads to fertilization in mammals, the two partners first meet head-to-zona. The subsequent of steps, which appears applicable to hamster (7,8,13) and mouse (16), if not to guinea pig (11), is the following: binding of capacitated sperm to tje zona; sperm acrosome reaction at the zone surface; sperm penetration of the zona; and gamete membrane fusion. It is the first two of these reactions in mouse gametes which we have examining in our studies; this paper presents a brief account of our findings and interpretations.
4 citations
01 Jan 1983
TL;DR: This work attempted to reveal surface membrane structures of the boar and rabbit spermatozoa by deep-etching and rotary shadowing, and obtained new findings that gathered particles of 15 nm in diameter with subunits appear on the ES face of the postacrosomal region of the Boar sperm.
Abstract: Specialized structures of the sperm membranes have been studied by surface replica and freeze-fracture (1–5). Domains of the sperm plasma membrane are also reported (2, 5). We attempted to reveal surface membrane structures of the boar and rabbit spermatozoa by deep-etching and rotary shadowing, and obtained new findings that gathered particles of 15 nm in diameter with subunits appear on the ES face of the postacrosomal region of the boar sperm.